PURIFICATION AND CHARACTERIZATION OF THE INVITRO ACTIVITY OF I-SCE-I, A NOVEL AND HIGHLY SPECIFIC ENDONUCLEASE ENCODED BY A GROUP-I INTRON

被引：140

作者：

MONTEILHET, C ^{[1
]}

PERRIN, A ^{[1
]}

THIERRY, A ^{[1
]}

COLLEAUX, L ^{[1
]}

DUJON, B ^{[1
]}

机构：

[1] INST PASTEUR,UNITE GENET MOLEC LEVURES,25 RUE DOCTEUR ROUX,F-75724 PARIS 15,FRANCE

来源：

NUCLEIC ACIDS RESEARCH | 1990年 / 18卷 / 06期

关键词：

D O I：

10.1093/nar/18.6.1407

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Group I intron encoded proteins represent a novel class of site specific double strand endonucleases. The endonuclease activity of this class of proteins has been first demonstrated in vivo for I-Sce I which is encoded by a mitochondrial intron of Saccharomyces cerevisiae. Assays using crude cell extracts have shown that I-Sce I can be used in vitro as a restriction endonuclease potentially useful for recombinant DNA technology owing to its large recognition sequence (18 nucleotides). We report here the purification and the first detailed analysis of the in vitro activity and properties of I-Sce I. © 1990 Oxford University Press.

引用

页码：1407 / 1413

页数：7

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