A CONTINGENT REPLICATION ASSAY FOR THE DETECTION OF PROTEIN PROTEIN INTERACTIONS IN ANIMAL-CELLS

被引：48

作者：

VASAVADA, HA ^{[1
]}

GANGULY, S ^{[1
]}

GERMINO, FJ ^{[1
]}

ZHEN, XW ^{[1
]}

WEISSMAN, SM ^{[1
]}

机构：

[1] YALE UNIV,SCH MED,DEPT MED,NEW HAVEN,CT 06510

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 23期

关键词：

SIMIAN VIRUS-40 LARGE TUMOR ANTIGEN; GAL4; PROTEIN; HERPES SIMPLEX VIRUS PROTEIN VP16; PLASMID R64; FOS-JUN COMPLEX;

D O I：

10.1073/pnas.88.23.10686

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have developed a sensitive and rapid assay system termed the contingent replication assay (CRA) for selecting cDNAs with desired functional properties from a cDNA library. The system functions in animal cells and permits enrichment of the desired cDNA in small-scale and convenient experiments. The assay can be used for the enrichment of proteins that activate transcription from conditional enhancers, bind to specific DNA sequences, or interact with target proteins of interest. In this communication we report the application of this assay to study protein-protein interactions in animal cells.

引用

页码：10686 / 10690

页数：5

共 29 条

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[2]

ARUFFO A, 1987, NATURE, V329, P840

[3]

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