THE USE OF TANDEM MASS-SPECTROMETRY FOR THE DIFFERENTIATION OF BILE-ACID ISOMERS AND FOR THE IDENTIFICATION OF BILE-ACIDS IN BIOLOGICAL EXTRACTS

被引：11

作者：

ECKERS, C

NEW, AP

EAST, PB

HASKINS, NJ

机构：

[1] Smith Kline and French Research Ltd., Welwyn, Hertfordshire, AL6 9AR, Smith Kline and French Research Ltd. The Frythe

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1990年 / 4卷 / 10期

关键词：

D O I：

10.1002/rcm.1290041024

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Tandem mass spectrometric (MS/MS) techniques have been widely used for the differentiation of isomeric compounds, since their spectra may show differences sufficient to distinguish between them. There are several different ways by which the MS/MS data can be obtained depending on the energies of the ions and the collisions. In this paper MS/MS spectra have been obtained for a group of isomeric bile acids using: 1, low‐energy ions and low‐energy collisions in a triple quadrupole mass spectrometer by liquid chromatography/MS/MS; 2, high‐energy ions and low‐energy collisions in a hybrid mass spectrometer by fast‐atom bombardment MS/MS. Liquid chromatography combined with tandem mass spectrometry (LC/MS/MS) has also been used to identify the bile acids present in biological matrices such as bile extracts. Copyright © 1990 John Wiley & Sons Limited

引用

页码：449 / 453

页数：5

共 12 条

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