ACTIVATED CD4+ AND CD8+ LYMPHOCYTES-T IN NEWLY DIAGNOSED TYPE-1 DIABETES - A PROSPECTIVE-STUDY

被引：13

作者：

BUSCHARD, K

DAMSBO, P

ROPKE, C

机构：

[1] Bartholin Institute, Copenhagen, Kommunehospitalet

[2] Hvidöre Hospital, Klampenborg

[3] Institute of Medical Anatomy, Department A, Panum Institute, University of Copenhagen

来源：

DIABETIC MEDICINE | 1990年 / 7卷 / 02期

关键词：

Activated T‐lymphocytes; Diagnosis; Flow‐cytometer; Helper T‐cells; Prospective study; Suppressor/cytotoxic T‐cells; Type; 1; diabetes;

D O I：

10.1111/j.1464-5491.1990.tb01347.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Peripheral lymphocytes from 13 Type 1 diabetic patients (age 28 ± 11 (±SD) years) were studied at diagnosis, and 1 month and 7 months later. The lymphocytes were labelled with phycoerythrin‐conjugated anti‐HLA‐DR antibody and/or fluorescein‐conjugated OKT4 or OKT8 antibody, and then studied using a double channel flow‐cytometer. The overall percentage of activated T‐lymphocytes (CD4+ + CD8+ cells) was (mean ± SE) 3.1 ± 0.5% at diagnosis, 2.1 ± 0.3% at 1 month and 2.2 ± 0.5% at 7 months test. The percentages at diagnosis and 1 month were higher than in 12 healthy control subjects (1.0 ± 0.2%, p < 0.001 and p < 0.01, respectively). Among the CD4+ cells the percentage of activated cells fell from diagnosis (2.8 ± 0.7%), to 1 month (1.5 ± 0.3%, p < 0.05), and 7 months (1.5 ± 0.4%, p < 0.05), whereas among the CD8+ cells they remained unchanged (3.3 ± 0.6, 2.5 ± 0.5 and 3.1 ± 0.6%). The percentage of activated CD4+ cells at diagnosis, and of CD8+ cells at all times, were higher (p < 0.02) than in control subjects (CD4+, 0.8 ± 0.2%; CD8+, 1.2 ± 0.2%). In conclusion, the activated T‐lymphocytes present in Type 1 diabetic patients at diagnosis are equally increased among CD4+ and CD8+ cells, but after 7 months the increase is predominantly in CD8+ cells. 1990 Diabetes UK

引用

页码：132 / 136

页数：5

共 21 条

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