VIP STIMULATION OF POLARIZED MACROMOLECULE SECRETION IN CULTURED CHICK EMBRYONIC RETINAL-PIGMENT EPITHELIUM

被引：17

作者：

KOH, SWM

机构：

[1] Department of Ophthalmology, University of Maryland at Baltimore, Baltimore

来源：

EXPERIMENTAL CELL RESEARCH | 1991年 / 197卷 / 01期

关键词：

D O I：

10.1016/0014-4827(91)90472-7

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Vasoactive intestinal peptide (VIP) stimulated macromolecule secretion at the apical membranes of the chick embryonic retinal pigment epithelium cultured on permeable supports in a time- and concentration-dependent manner. VIP stimulated secretion of molecules with MW of 80, 74, 70, 60, 42, 35, 24, 20, and 14 kDa. A 1.9- to 2.6-fold stimulation in secretion of molecules with MW > 10 kDa precipitable by 10% trichloroacetic acid was observed after treatment with 1 μM VIP for 15 min. The effect of 1 μM VIP was mimicked by 10 μM dibutyryl cyclic AMP and attenuated by dopamine (1 × 10-1M), while colchicine, β-lumicolchicine, and monensin, all at 1 μM, had no effect. © 1991 Academic Press, Inc. All rights of reproduction in any form reserved.

引用

页码：1 / 7

页数：7

共 27 条

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[2]

CUNHAVAZ JF, 1980, NATO ADV STUDY I A, V32

[3] PROGLUCAGON GENE-EXPRESSION IS REGULATED BY A CYCLIC AMP-DEPENDENT PATHWAY IN RAT INTESTINE [J].