DETECTION AND ANALYSIS OF HEPATITIS-C VIRUS BY A COMBINED RT-PCR METHOD - VARIATION IN THE 5' NONCODING REGION OF THE VIRAL GENOME

被引：3

作者：

KARACHRISTOS, A

LINARDOPOULOS, S

ERGAZAKI, M

SPANDIDOS, DA

机构：

[1] UNIV CRETE,SCH MED,CLIN VIROL LAB,IRAKLION,GREECE

[2] NATL HELLEN RES FDN,MOLEC ONCOL & BIOTECHNOL LAB,ATHENS,GREECE

[3] BEATSON INST CANC RES,GLASGOW G61 1BD,LANARK,SCOTLAND

来源：

JOURNAL OF MEDICAL MICROBIOLOGY | 1995年 / 42卷 / 05期

关键词：

D O I：

10.1099/00222615-42-5-367

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A combined reverse transcription-polymerase chain reaction (RT-PCR) method was employed for the detection of hepatitis C virus (HCV) RNA in serum from patients with chronic active hepatitis, with primers corresponding to the 5' non-coding region. The diagnosis was based on serological and biochemical methods and on liver biopsy. HCV-RNA was detected in 27 (90%) of 30 sera examined. The nucleotide sequence of PCR-amplified HCV cDNAs (256 bp) was determined from five specimens and heterogeneity varying between 0.58% and 2.89% among the clinical samples and the prototype HCV-1 was found.

引用

收藏

页码：367 / 371

页数：5

相关论文

共 27 条

[1] DETECTION OF ANTIBODY TO HEPATITIS-C VIRUS IN PROSPECTIVELY FOLLOWED TRANSFUSION RECIPIENTS WITH ACUTE AND CHRONIC NON-A-HEPATITIS, NON-B-HEPATITIS [J].

ALTER, HJ ;

PURCELL, RH ;

SHIH, JW ;

MELPOLDER, JC ;

HOUGHTON, M ;

CHOO, QL ;

KUO, G .

NEW ENGLAND JOURNAL OF MEDICINE, 1989, 321 (22) :1494-1500

[2] SECONDARY STRUCTURE OF THE 5' NONTRANSLATED REGIONS OF HEPATITIS-C VIRUS AND PESTIVIRUS GENOMIC RNAS [J].

BROWN, EA ;

ZHANG, HC ;

PING, LH ;

LEMON, SM .

NUCLEIC ACIDS RESEARCH, 1992, 20 (19) :5041-5045

[3] IMPORTANCE OF PRIMER SELECTION FOR THE DETECTION OF HEPATITIS-C VIRUS-RNA WITH THE POLYMERASE CHAIN-REACTION ASSAY [J].

BUKH, J ;

PURCELL, RH ;

MILLER, RH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (01) :187-191

[4] AT LEAST 12 GENOTYPES OF HEPATITIS-C VIRUS PREDICTED BY SEQUENCE-ANALYSIS OF THE PUTATIVE E1-GENE OF ISOLATES COLLECTED WORLDWIDE [J].

BUKH, J ;

PURCELL, RH ;

MILLER, RH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (17) :8234-8238

[5]

BUKH J, 1991, P NATL ACAD SCI USA, V89, P4942

[6] IMPACT OF SPECIMEN HANDLING AND STORAGE ON DETECTION OF HEPATITIS-C VIRUS-RNA [J].

BUSCH, MP ;

WILBER, JC ;

JOHNSON, P ;

TOBLER, L ;

EVANS, CS .

TRANSFUSION, 1992, 32 (05) :420-425

[7] ANALYSIS OF A NEW HEPATITIS-C VIRUS TYPE AND ITS PHYLOGENETIC RELATIONSHIP TO EXISTING VARIANTS [J].

CHAN, SW ;

MCOMISH, F ;

HOLMES, EC ;

DOW, B ;

PEUTHERER, JF ;

FOLLETT, E ;

YAP, PL ;

SIMMONDS, P .

JOURNAL OF GENERAL VIROLOGY, 1992, 73 :1131-1141

[8] THE TAIWANESE HEPATITIS-C VIRUS GENOME - SEQUENCE DETERMINATION AND MAPPING THE 5' TERMINI OF VIRAL GENOMIC AND ANTIGENOMIC RNA [J].

CHEN, PJ ;

LIN, MH ;

TAI, KF ;

LIU, PC ;

LIN, CJ ;

CHEN, DS .

VIROLOGY, 1992, 188 (01) :102-113

[9] ISOLATION OF A CDNA CLONE DERIVED FROM A BLOOD-BORNE NON-A, NON-B VIRAL-HEPATITIS GENOME [J].

CHOO, QL ;

KUO, G ;

WEINER, AJ ;

OVERBY, LR ;

BRADLEY, DW ;

HOUGHTON, M .

SCIENCE, 1989, 244 (4902) :359-362

[10] GENETIC ORGANIZATION AND DIVERSITY OF THE HEPATITIS-C VIRUS [J].

CHOO, QL ;

RICHMAN, KH ;

HAN, JH ;

BERGER, K ;

LEE, C ;

DONG, C ;

GALLEGOS, C ;

COIT, D ;

MEDINASELBY, A ;

BARR, PJ ;

WEINER, AJ ;

BRADLEY, DW ;

KUO, G ;

HOUGHTON, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (06) :2451-2455