YIELD OF FLUORESCENCE FROM INDOCYANINE GREEN IN PLASMA AND FLOWING BLOOD

被引：42

作者：

VANDENBIESEN, PR

JONGSMA, FH

TANGELDER, GJ

SLAAF, DW

机构：

[1] UNIV LIMBURG,CARDIOVASC RES INST MAASTRICHT,DEPT BIOPHYS,6200 MD MAASTRICHT,NETHERLANDS

[2] UNIV LIMBURG,ACAD HOSP MAASTRICHT,DEPT OPHTHALMOL,6200 MD MAASTRICHT,NETHERLANDS

[3] UNIV LIMBURG,CARDIOVASC RES INST MAASTRICHT,DEPT PHYSIOL,6200 MD MAASTRICHT,NETHERLANDS

来源：

ANNALS OF BIOMEDICAL ENGINEERING | 1995年 / 23卷 / 04期

关键词：

RETINAL VESSELS; INDOCYANINE GREEN ANGIOGRAPHY; SHEAR RATE; HEMATOCRIT; YIELD OF FLUORESCENCE; SCANNING LASER OPHTHALMOSCOPE;

D O I：

10.1007/BF02584447

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The purpose of this work was to obtain more quantitative knowledge about the yield of fluorescence from retinal vessels during indocyanine green angiography (ICG). The yield of fluorescence from blood was investigated for various shear rates, concentrations of ICG, and layer thicknesses. Measurements were performed in vitro on samples of human blood in a cone-plate shear chamber using frontal illumination as in scanning laser angiography. In blood and in plasma, the yield of fluorescence of ICG increased with concentration up to 0.05 and 0.1 mg/ml, respectively. At higher concentrations, the yield decreased for all layer thicknesses. For increasing layer thicknesses, both in plasma and in blood, the yield of ICG fluorescence increased nonlinearly for concentrations higher than 0.012 mg/ml. Saturation occurred for layers thicker than 200 mu m in combination with ICG concentrations of 0.4 mg/ml and higher. Application of shear rates within the physiological range of the microcirculation (88/sec and 528/sec) increased the yield of fluorescence from the blood sample compared with stasis. The high transparency of blood for the excitation and emission light of ICG that was demonstrated will lead to superposition of fluorescence from superficial and deeper layers. This superposition precludes quantitative indocyanine angiography of ocular vessels.

引用

页码：475 / 481

页数：7

共 16 条

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