METALLOPORPHYRIN SPECIATION BY LIQUID-CHROMATOGRAPHY AND INDUCTIVELY-COUPLED PLASMA-MASS SPECTROMETRY

被引：15

作者：

KUMAR, U

DORSEY, JG

CARUSO, JA

EVANS, EH

机构：

[1] UNIV CINCINNATI,DEPT CHEM,CINCINNATI,OH 45221

[2] UNIV PLYMOUTH,DEPT ENVIRONM SCI,PLYMOUTH PL4 8AA,DEVON,ENGLAND

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 1994年 / 32卷 / 07期

关键词：

D O I：

10.1093/chromsci/32.7.282

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Inductively coupled plasma-mass spectrometry is investigated for the detection of metalloporphyrins (cobalt protoporphyrin, hemin, and zinc protoporphyrin) separated by liquid chromatography. A Hypersil SAS C1column with mobile phase containing 68% methanol at a pH of 4.5 is used. The detection limits obtained are in the nanogram range for cobalt and zinc protoporphyrins, whereas for hemin, detection limits are in the microgram range. The accuracy of the method is evaluated by determining zinc protoporphyrin from the whole blood of a lead-poisoned patient. © 1994 Oxford University Press.

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页码：282 / 285

页数：4

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