RAPID GENOTYPING SHOWS THE ABSENCE OF CROSS-CONTAMINATION IN ENTEROBACTER-CLOACAE NOSOCOMIAL INFECTIONS

被引：32

作者：

BINGEN, E ^{[1
]}

DENAMUR, E ^{[1
]}

LAMBERTZECHOVSKY, N ^{[1
]}

BRAHIMI, N ^{[1
]}

ELLAKANY, M ^{[1
]}

ELION, J ^{[1
]}

机构：

[1] HOP ROBERT DEBRE,BIOCHIM GENET LAB,F-75019 PARIS,FRANCE

来源：

JOURNAL OF HOSPITAL INFECTION | 1992年 / 21卷 / 02期

关键词：

ENTEROBACTER-CLOACAE; GENOTYPING; HOSPITAL OUTBREAK;

D O I：

10.1016/0195-6701(92)90028-K

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Restriction fragment length polymorphism analysis (RFLP) of total DNA and rDNA regions was used for the epidemiological evaluation of 10 Enterobacter cloacae nosocomial isolates obtained from nine patients in our hospital. Five of these patients were hospitalized during overlapping periods, thus raising the question of cross-contamination. A single biochemical pattern and antibiotic susceptibility profile was observed for all isolates but one. In contrast, based on the results of total DNA and rDNA RFLP patterns, the genetic unrelatedness of the isolates was clearly shown, thus excluding a common source of contamination or patient-to-patient transfer. © 1992.

引用

页码：95 / 101

页数：7

共 15 条

[1] ANALYSIS OF DNA RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM EXTENDS THE EVIDENCE FOR BREAST-MILK TRANSMISSION IN STREPTOCOCCUS-AGALACTIAE LATE-ONSET NEONATAL INFECTION [J].

BINGEN, E ;

DENAMUR, E ;

LAMBERTZECHOVSKY, N ;

AUJARD, Y ;

BRAHIMI, N ;

GESLIN, P ;

ELION, J .

JOURNAL OF INFECTIOUS DISEASES, 1992, 165 (03) :569-573

[2] MOTHER-TO-INFANT VERTICAL TRANSMISSION AND CROSS-COLONIZATION OF STREPTOCOCCUS-PYOGENES CONFIRMED BY DNA RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM ANALYSIS [J].

BINGEN, E ;

DENAMUR, E ;

LAMBERTZECHOVSKY, N ;

BOISSINOT, C ;

BRAHIMI, N ;

AUJARD, Y ;

BLOT, P ;

ELION, J .

JOURNAL OF INFECTIOUS DISEASES, 1992, 165 (01) :147-150

[3] EVIDENCE FOR THE GENETIC UNRELATEDNESS OF NOSOCOMIAL VANCOMYCIN-RESISTANT ENTEROCOCCUS-FAECIUM STRAINS IN A PEDIATRIC HOSPITAL [J].

BINGEN, EH ;

DENAMUR, E ;

LAMBERTZECHOVSKY, NY ;

ELION, J .

JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (09) :1888-1892

[4] DNA RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM DIFFERENTIATES CROSSED FROM INDEPENDENT INFECTIONS IN NOSOCOMIAL XANTHOMONAS-MALTOPHILIA BACTEREMIA [J].

BINGEN, EH ;

DENAMUR, E ;

LAMBERTZECHOVSKY, NY ;

BOURDOIS, A ;

MARIANIKURKDJIAN, P ;

CEZARD, JP ;

NAVARRO, J ;

ELION, J .

JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (07) :1348-1350

[5]

BODEY GP, 1991, REV INFECT DIS, V13, P550

[6] OUTBREAK OF GUT COLONIZATION BY PSEUDOMONAS-AERUGINOSA IN IMMUNOCOMPROMISED CHILDREN UNDERGOING TOTAL DIGESTIVE DECONTAMINATION - ANALYSIS BY PULSED-FIELD ELECTROPHORESIS [J].

BOUKADIDA, J ;

DEMONTALEMBERT, M ;

GAILLARD, JL ;

GOBIN, J ;

GRIMONT, F ;

GIRAULT, D ;

VERON, M ;

BERCHE, P .

JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (09) :2068-2071

[7] PATIENTS ENDOGENOUS FLORA AS THE SOURCE OF NOSOCOMIAL ENTEROBACTER IN CARDIAC-SURGERY [J].

FLYNN, DM ;

WEINSTEIN, RA ;

NATHAN, C ;

GASTON, MA ;

KABINS, SA .

JOURNAL OF INFECTIOUS DISEASES, 1987, 156 (02) :363-368

[8] RAPID METHODS IN BACTERIAL-DNA FINGERPRINTING [J].

FORBES, KJ ;

BRUCE, KD ;

JORDENS, JZ ;

BALL, A ;

PENNINGTON, TH .

JOURNAL OF GENERAL MICROBIOLOGY, 1991, 137 :2051-2058

[9]

GALLAGHER PG, 1990, REV INFECT DIS, V12, P808

[10] COMPARISON OF RIBOTYPING WITH CONVENTIONAL METHODS FOR THE TYPE IDENTIFICATION OF ENTEROBACTER-CLOACAE [J].

GARAIZAR, J ;

KAUFMANN, ME ;

PITT, TL .

JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (07) :1303-1307

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