INOSITOL IS A CONSTITUENT OF DETERGENT-SOLUBILIZED IMMUNOAFFINITY-PURIFIED RAT-LIVER 5'-NUCLEOTIDASE

被引:24
作者
BAILYES, EM [1 ]
FERGUSON, MAJ [1 ]
COLACO, CALS [1 ]
LUZIO, JP [1 ]
机构
[1] UNIV DUNDEE,DEPT BIOCHEM,DUNDEE DD1 4HN,SCOTLAND
关键词
D O I
10.1042/bj2650907
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
myo-Inositol analysis of detergent-solubilized immunoaffinity-purified rat liver 5'-nucleotidase showed the presence of 1 mol of myo-inositol/mol of enzyme monomer. This provides unequivocal evidence that the ectoenzyme 5'-nucleotidase is attached to liver membranes by a glycosyl-phosphatidylinositol lipid anchor.
引用
收藏
页码:907 / 909
页数:3
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