SHORT CHAIN FLAVOR ESTERS SYNTHESIS BY MICROBIAL LIPASES

The peparative synthesis of 35 short chain flavour esters by lipases from Mucor miehi, Aspergillus sp., Candida rugosa and Rhizopus arrhizus was investigated in organic media. Acetic, propionic, butyric, valeric and caproic acids, as well as methanol, ethanol, butanol, i-pentanol, hexanol, citronellol and geraniol were used as substrates. Most of the esters were synthesized in good yield by at least one of the lipase preparations tested. Different conversion yields were observed according to the lipase specificity toward the acid or the alcohol moiety of the ester. Methyl- and ethyl acetates were also produced by changing the organic solvent. Enzymatic catalysis in organic solvent is thought to be a valuable method for preparative synthesis of flavour esters. © 1990 Science and Technology Letters.