END-FILLING OF AN OLIGONUCLEOTIDE DUPLEX CONTAINING AN MDBP SITE IN THE HUMAN HSP70 PROMOTER INHIBITS PROTEIN-DNA COMPLEX-FORMATION

被引：1

作者：

ASIEDU, CK

SUPAKAR, PC

EHRLICH, M

机构：

[1] Department of Biochemistry, Tulane Medical School, New Orleans

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 178卷 / 03期

关键词：

D O I：

10.1016/0006-291X(91)90980-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A site from the promoter region of the human hsp70 gene binds with a high affinity to the ubiquitous mammalian protein called methylated DNA-binding protein (MDBP) when it is present in a CpG-methylated oligonucleotide duplex with only 14 base-pairs. Binding to this site is dependent upon CpG methylation. Surprisingly, when the same methylated sequence is present in a duplex that has 22 or more base-pairs, binding to this protein is greatly inhibited. Such a requirement for a short duplex region is seen only in certain of the cytosine methylation-dependent binding sites for this protein and is proposed to reflect differences in the conformation of the duplex due to small differences in the nucleotide sequence. © 1991.

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页码：927 / 933

页数：7

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