PURIFICATION, CHARACTERIZATION, GENE CLONING, AND SEQUENCING OF A NEW BETA-GLUCOSIDASE FROM BACILLUS-CIRCULANS SUBSP ALKALOPHILUS

被引：48

作者：

PAAVILAINEN, S ^{[1
]}

HELLMAN, J ^{[1
]}

KORPELA, T ^{[1
]}

机构：

[1] CTR BIOTECHNOL,SF-20521 TURKU,FINLAND

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1993年 / 59卷 / 03期

关键词：

D O I：

10.1128/AEM.59.3.927-932.1993

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An intracellular beta-glucosidase was purified from cell extracts of Bacillus circulans subsp. alkalophilus by NAD affinity and high-performance anion-exchange chromatographies. The enzyme was active against a wide range of aryl-beta-glucosides and beta-linked disaccharides. The structural gene for beta-glucosidase was cloned in Escherichia coli. The beta-glucosidase gene consisted of an open reading frame of 1,350 bp encoding a protein of 450 amino acids with a calculated M(r) of 51,303. The enzyme exhibited from 45 to 66% identity with five bacterial beta-glucosidases.

引用

页码：927 / 932

页数：6

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