THE WHITE GENE OF CERATITIS-CAPITATA - A PHENOTYPIC MARKER FOR GERMLINE TRANSFORMATION

被引:81
作者
ZWIEBEL, LJ
SACCONE, G
ZACHAROPOULOU, A
BESANSKY, NJ
FAVIA, G
COLLINS, FH
LOUIS, C
KAFATOS, FC
机构
[1] EUROPEAN MOLEC BIOL LAB, D-69117 HEIDELBERG, GERMANY
[2] HARVARD UNIV, DEPT CELLULAR & DEV BIOL, CAMBRIDGE, MA 02138 USA
[3] FORTH, INST MOLEC BIOL & BIOTECHNOL, CRETE, GREECE
[4] UNIV NAPLES FEDERICO II, DIPARTIMENTO GENET BIOL GEN & MOLEC, NAPLES, ITALY
[5] UNIV PATRAS, DEPT BIOL, PATRAI, GREECE
[6] CTR DIS CONTROL & PREVENT, DIV PARASIT DIS, ATLANTA, GA 30341 USA
关键词
D O I
10.1126/science.270.5244.2005
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Reliable germline transformation is required for molecular studies and ultimately for genetic control of economically important insects, such as the Mediterranean fruit fly (medfly) Ceratitis capitata. A prerequisite for the establishment and maintenance of transformant lines is selectable or phenotypically dominant markers. To this end, a complementary DNA clone derived from the medfly white gene was isolated, which showed substantial similarity to white genes in Drosophila melanogaster and other Diptera. It is correlated with a spontaneous mutation causing white eyes in the medfly and can be used to restore partial eye color in transgenic Drosophila carrying a null mutation in the endogenous white gene.
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页码:2005 / 2008
页数:4
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