SARCOPLASMIC-RETICULUM FROM FLOUNDER MUSCLE HAVING IMPROVED LIPID PEROXIDATIVE ACTIVITY

被引：25

作者：

BORHAN, M ^{[1
]}

SHEWFELT, RL ^{[1
]}

HULTIN, HO ^{[1
]}

机构：

[1] UNIV MASSACHUSETTS, DEPT FOOD SCI & NUTR, MARINE FOODS LAB, MARINE STN, MASSACHUSETTS AGR EXPT STN, GLOUCESTER, MA 01930 USA

来源：

ANALYTICAL BIOCHEMISTRY | 1984年 / 137卷 / 01期

关键词：

D O I：

10.1016/0003-2697(84)90346-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A procedure for the isolation of sarcoplasmic reticulum from winter flounder (P. americanus) resulted in a fraction with a specific activity of lipid-peroxidation 2-3 times that of previous preparations. Good stability of the NADH-dependent lipid peroxidative activity was achieved. There appeared to be minimal contamination of the preparation with lysosomes and mitochondria. The flounder sarcoplasmic reticulum was highly active with respect to ATPase and calcium uptake. The membrane fraction contained 43% lipid and 57% protein; 60% of the lipids were phospholipids. Phosphatidylcholine was the major phospholipid present.

引用

页码：58 / 65

页数：8

共 35 条

[1] EFFECTS OF ATP ON INTERACTION OF CA++, MG++, AND K+ WITH FRAGMENTED SARCOPLASMIC RETICULUM ISOLATED FROM RABBIT SKELETAL MUSCLE [J].