NUCLEAR-DNA VARIABILITY WITHIN PISUM-SATIVUM (LEGUMINOSAE) - CYTOPHOTOMETRIC ANALYSES

被引:25
作者
CAVALLINI, A
NATALI, L
机构
[1] Sezione di Genetica, Dipartimento di Biologia delle Piante Agrarie della Università di Pisa, Pisa, I-56100
关键词
Angiosperms; Cytophotometry; DNA variability; Leguminosae; nuclear DNA content; Pisum sativum;
D O I
10.1007/BF00940861
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The cultivars or experimental lines of Pisum sativum were analyzed cytophotometrically for nuclear DNA content of early prophases after ethanol-acetic acid fixation. Wide variability was found (from 3.93 to 5.07pg per haploid nucleus). This result was confirmed by the cytophotometric analysis of interphase nuclei isolated from leaf tissues fixed in formalin. Analysis of interphase nuclei at different thresholds of optical density showed that certain chromatin fractions are involved in the variations. © 1990 Springer-Verlag.
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页码:179 / 185
页数:7
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