BIOCHEMICAL-CHARACTERIZATION OF HERPES-SIMPLEX VIRUS TYPE-1-IMMUNOSTIMULATING COMPLEXES (ISCOMS) - A MULTI-GLYCOPROTEIN STRUCTURE

被引：15

作者：

ERTURK, M ^{[1
]}

JENNINGS, R ^{[1
]}

PHILLPOTTS, RJ ^{[1
]}

POTTER, CW ^{[1
]}

机构：

[1] CTR APPL MICROBIOL & RES,SALISBURY,WILTS,ENGLAND

来源：

VACCINE | 1991年 / 9卷 / 09期

关键词：

HSV-1; GLYCOPROTEINS; ISCOMS; VACCINE;

D O I：

10.1016/0264-410X(91)90193-A

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The preparation and characterization of an immunostimulating complex (ISCOM) preparation containing several HSV-1 glycoproteins, including the major glycoproteins B and D is described. The multi-glycoprotein HSV-1 ISCOM preparation was obtained from a gradient-purified aqueous HSV-1 antigen preparation following extraction from infected cells using a zwitterionic detergent. With polyclonal and monoclonal antibodies to HSV-1 glycoproteins in enzyme-linked immunosorbent assay, SDS-polyacrylamide gel electrophoresis and radioimmunoprecipitation techniques, the HSV-1 ISCOM preparation was shown to contain glycoproteins B, C, D, E, H and I, although further, additional proteins were also present. The DNA content of HSV-1 ISCOMs was determined using a H-3-thymidine labelling method. The protein and DNA contents of the HSV-1 ISCOM preparation are discussed with reference to the potentialities of the preparation as a vaccine for use in human beings.

引用

页码：668 / 674

页数：7

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[1] FORMATION AND CHARACTERIZATION OF FELV ISCOMS [J].