EVALUATION OF SEQUENCE-TAGGED-SITE PCR PRODUCTS AS MOLECULAR MARKERS IN WHEAT

被引:120
作者
TALBERT, LE
BLAKE, NK
CHEE, PW
BLAKE, TK
MAGYAR, GM
机构
[1] Department of Plant and Soil Science, Montana State University, Bozeman, Montana
关键词
WHEAT; MOLECULAR; MARKERS; BREEDING;
D O I
10.1007/BF00221130
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
The polymerase chain reaction (PCR) is an attractive technique for many genome mapping and characterization projects. One PCR approach which has been evaluated involves the use of randomly amplified polymorphic DNA (RAPD). An alternative to RAPDs is the sequence-tagged-site (STS) approach, whereby PCR primers are designed from mapped low-copy-number sequences. In this study, we sequenced and designed primers from 22 wheat RFLP clones in addition to testing 15 primer sets that had been previously used to amplify DNA sequences in the barley genome. Our results indicated that most of the primers amplified sequences that mapped to the expected chromosomes in wheat. Additionally, 9 of 16 primer sets tested revealed polymorphisms among 20 hexaploid wheat genotypes when PCR products were digested with restriction enzymes. These results suggest that the STS-based PCR analysis will be useful for generation of informative molecular markers in hexaploid wheat.
引用
收藏
页码:789 / 794
页数:6
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