RAT-LIVER MITOCHONDRIAL ADP-RIBOSE PYROPHOSPHATASE IN THE MATRIX SPACE WITH LOW K-M FOR FREE ADP-RIBOSE

被引：26

作者：

BERNET, D ^{[1
]}

PINTO, RM ^{[1
]}

COSTAS, MJ ^{[1
]}

CANALES, J ^{[1
]}

CAMESELLE, JC ^{[1
]}

机构：

[1] UNIV EXTREMADURA, FAC MED, DEPT BIOQUIM & BIOL MOLEC & GENET, E-06080 BADAJOZ, SPAIN

来源：

BIOCHEMICAL JOURNAL | 1994年 / 299卷

关键词：

D O I：

10.1042/bj2990679

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A study involving markers of subcellular and submitochondrial fractions, gradient centrifugation, latency measurements and extraction with digitonin, demonstrates the association of a specific ADP-ribose pyrophosphatase with rat liver mitochondria and its localization in the matrix space. The enzyme hydrolyses ADP-ribose to AMP, with a K-m of 2-3 mu M. The results support the occurrence of a specific turnover pathway for the ADP-ribose and its relevance in mitochondria.

引用

页码：679 / 682

页数：4

共 31 条

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