DETECTION OF THE SURFACE-EXPOSED 18-KILODALTON BINDING-PROTEIN IN CHLAMYDIA-TRACHOMATIS BY IMMUNOGOLD STAINING

被引：10

作者：

GRAY, GJ

KAUL, R

SHERBURNE, R

WENMAN, WM

机构：

[1] UNIV ALBERTA,DEPT MED MICROBIOL,EDMONTON T6G 2R7,ALBERTA,CANADA

[2] UNIV ALBERTA,DEPT PEDIAT,EDMONTON T6G 2R7,ALBERTA,CANADA

来源：

JOURNAL OF BACTERIOLOGY | 1990年 / 172卷 / 06期

关键词：

D O I：

10.1128/jb.172.6.3524-3528.1990

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Dot-blot analysis of Chlamydia trachomatis elementary bodies (EBs) with monospecific polyclonal antibodies demonstrated that the 18-kilodalton binding protein is surface exposed. Immunoelectron microscopy with whole serovar L2 EBs and ultrathin sections confirmed this finding. In addition, only the extracellular EBs and not the intracellular reticulate bodies were labeled with immunogold.

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页码：3524 / 3528

页数：5

相关论文

共 17 条

[1] MAPPING ANTIGENIC DOMAINS EXPRESSED BY CHLAMYDIA-TRACHOMATIS MAJOR OUTER-MEMBRANE PROTEIN GENES [J].

BAEHR, W ;

ZHANG, YX ;

JOSEPH, T ;

SU, H ;

NANO, FE ;

EVERETT, KDE ;

CALDWELL, HD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (11) :4000-4004

[2] ROLE OF DISULFIDE BONDING IN OUTER-MEMBRANE STRUCTURE AND PERMEABILITY IN CHLAMYDIA-TRACHOMATIS [J].

BAVOIL, P ;

OHLIN, A ;

SCHACHTER, J .

INFECTION AND IMMUNITY, 1984, 44 (02) :479-485

[3] PURIFICATION AND PARTIAL CHARACTERIZATION OF THE MAJOR OUTER-MEMBRANE PROTEIN OF CHLAMYDIA-TRACHOMATIS [J].

CALDWELL, HD ;

KROMHOUT, J ;

SCHACHTER, J .

INFECTION AND IMMUNITY, 1981, 31 (03) :1161-1176

[4]

FAULK WP, 1971, IMMUNOCHEMISTRY, V8, P1081

[5] IDENTIFICATION AND PROPERTIES OF CHLAMYDIAL POLYPEPTIDES THAT BIND EUKARYOTIC CELL-SURFACE COMPONENTS [J].

HACKSTADT, T .

JOURNAL OF BACTERIOLOGY, 1986, 165 (01) :13-20

[6] EXPRESSION OF THE CHLAMYDIA-TRACHOMATIS MAJOR OUTER-MEMBRANE PROTEIN-ENCODING GENE IN ESCHERICHIA-COLI - ROLE OF THE 3' END IN MESSENGER-RNA STABILITY [J].

KAUL, R ;

DUNCAN, MJJ ;

GUEST, J ;

WENMAN, WM .

GENE, 1990, 87 (01) :97-103

[7] ULTRASTRUCTURAL-STUDY OF CHLAMYDIA-TRACHOMATIS SURFACE-ANTIGENS BY IMMUNOGOLD STAINING WITH MONOCLONAL-ANTIBODIES [J].

KUO, CC ;

CHI, EY .

INFECTION AND IMMUNITY, 1987, 55 (05) :1324-1328

[8]

KUO CC, 1977, NONGONOCOCCAL URETHR, P328

[9]

Newhall W. J., 1988, MICROBIOLOGY CHLAMYD, P47

[10]

OLMSTED JB, 1981, J BIOL CHEM, V256, P1955