IMMOBILIZED CELL BIOCATALYST ACTIVATION AND PSEUDO-STEADY-STATE BEHAVIOR - MODEL AND EXPERIMENT

被引：75

作者：

MONBOUQUETTE, HG ^{[1
]}

SAYLES, GD ^{[1
]}

OLLIS, DF ^{[1
]}

机构：

[1] N CAROLINA STATE UNIV, DEPT CHEM ENGN, RALEIGH, NC 27695 USA

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1990年 / 35卷 / 06期

关键词：

D O I：

10.1002/bit.260350608

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An intrinsic, unstructured model has been utilized to describethe startup dynamics of a continuous Caalginate‐immobilized Zymomonas mobilis (ATCC 10988) fermentation. This model predicts, at least qualitatively, transients in the fermenter effluent glucose, ethanol, and biomass concentrations as well as radial gradients in immobilized‐cell concentration and activity within the gelbiocatalysts. Predicted intrabiocatalyst gradients in immobilized‐cell specific growth rate were used to calculate the corresponding gradients in intracellular RNA level based on a reported linear relationship between the two. Mathematical simulations of immobilized biomass concentration profiles and RNA content were verified using a novel, scanning microfluorimetry technique. Copyright © 1990 John Wiley & Sons, Inc.

引用

页码：609 / 629

页数：21

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