SEQUENCE AND EXPRESSION OF RAT ICAM-1

被引：107

作者：

KITA, Y

TAKASHI, T

IIGO, Y

TAMATANI, T

MIYASAKA, M

HORIUCHI, T

机构：

[1] DAIICHI PHARMACEUT CO,TOKYO RES & DEV CTR,MOLEC BIOL RES LAB,KITA KASAI 1 CHOME,EDOGAWA KU,TOKYO 134,JAPAN

[2] DAIICHI PHARMACEUT CO,TOKYO RES & DEV CTR,EXPLORATORY RES LABS 3,TOKYO,JAPAN

[3] TOKYO METROPOLITAN INST MED SCI,DEPT IMMUNOL,TOKYO 113,JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1131卷 / 01期

关键词：

ADHESION MOLECULE; RAT ICAM-1; CDNA;

D O I：

10.1016/0167-4781(92)90107-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated cDNA clones-coding for rat intercellular adhesion molecule-1 (RICAM-1) from a cDNA library constructed from rat Ax cells stimulated with IL-1-beta using the mouse ICAM-1 cDNA as a hybridization probe. The RICAM-1 sequence shows 79.1% homology with mouse ICAM-1 and 55.6% homology with human ICAM-1 at the nucleic acid level. In order to examine the expression of RICAM-1 on Chinese hamster ovary (CHO) cells, we constructed the vector, pSV-RICAM1-nco. containing the SV40 promoter. Flowcytometric analysis showed that CHO-K1 cells transfected with pSV-RICAM1-neo expressed high amounts of RICAM-1 on their surfaces.

引用

页码：108 / 110

页数：3

共 12 条

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