ALLICIN-INDUCED HYPOTENSION IN RABBIT EYES

被引：16

作者：

CHU, TC ^{[1
]}

OGIDIGBEN, M ^{[1
]}

HAN, JC ^{[1
]}

POTTER, DE ^{[1
]}

机构：

[1] MOREHOUSE SCH MED,DEPT PHARMACOL & TOXICOL,720 WESTVIEW DR SW,ATLANTA,GA 30310

来源：

JOURNAL OF OCULAR PHARMACOLOGY | 1993年 / 9卷 / 03期

关键词：

D O I：

10.1089/jop.1993.9.201

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

The intent of this work was to examine the actions of allicin on 1) intraocular pressure (IOP) in normal and unilaterally sympathectomized (SX) rabbits; 2) cAMP accumulation in the rabbit iris-ciliary body(ICB) and cultured nonpigmented epithelial (NPE) ciliary body cells; and 3) H-3-norepinephrine (NE) release by calculating fractional tritium overflow in response to electrical field stimulation(EFS, 5 Hz, 12 V/cm) in isolated, perfused rabbit ICBs. Allicin, one of the active compounds produced by garlic, was evaluated on IOP and it was determined that allicin (1, 2.5, or 10 ug) , topically, but not the precursor, alliin (10 ug) , lowered the IOP unilaterally in normal rabbits. Allicin(10 mug) reduced the IOP by 6 +/- 1 mmHg (n=4) in normal rabbits at 2 hrs (maximum response) whereas no change occurred in sympathectomized rabbit eyes. Moreover, allicin (0.01, 0.1, or 1 uM) caused 40, 40, or 52% inhibition, respectively, of H-3-NE overflow in response to EFS. Isoproterenol (ISO, 1 uM) stimulated cAMP accumulation by 3.6 and 9 fold in isolated rabbit ICB and cultured NPE cells, respectively. Allicin(1 uM) had no effect on basal cAMP level while it inhibited ISO-stimulated cAMP accumulation by 40% and 23% in ICB and NPE cells, respectively. This study suggests that allicin lowered IOP, in part, by dual actions at the neuroeffector junction.

引用

页码：201 / 209

页数：9

共 26 条

[1]

Block E., The chemistry of garlic and onions, Sci. Amer., 252, pp. 114-119, (1985)

[2]

Bordia A., Effect of garlic on blood lipids in patients with coronary heart disease, Am. J. Clin. Nutr., 34, pp. 2100-2103, (1981)

[3]

Harenberg J., Giese C., Zimmermann R., Effect of dried garlic on blood coagulation, fibrinolysis, platelet aggregation and serum cholesterol levels in patients with hyperlipoproteinemia, Atherosclerosis, 74, pp. 247-249, (1988)

[4]

Dausch J.G., Nixon D.W., Garlic, A review of its relationship to malignant disease, Prevent. Med., 19, pp. 346-361, (1990)

[5]

Lau B.H.S., Yamasaki T., Gridley D.S., Garlic compounds modulate macrophage and T-lymphocyte functions, Mol. Biother., 3, pp. 103-107, (1991)

[6]

Stoll A., Seebeck E., Die Synthese des naturlichen alliins und seiner drei optisch aktiven isomerasen, Hei. Chim. Acta, 31, pp. 189-210, (1948)

[7]

Rowland J.M., Potter D.E., Reiter R.J., Circadian rhythm in intraocular pressure: a rabbit model, Curr. Eve Res., 1, pp. 169-173, (1981)

[8]

Burke J.A., Potter D.E., Ocular effects of a relatively selective at2 agonist (UK-14, 304-18) in cats, rabbits and monkeys, Curr. Eve Res., 5, pp. 665-676, (1986)

[9]

Mayeux P.R., Agrawal K.C., Tou J.-S.H., King B.T., Lippton H.L., Hyman A.L., Kadowitz P.J., McNamara D.B., The pharmacological effects of allicin, a constituent of garlic oil, Agents and Actions, 25, pp. 182-190, (1988)

[10]

Lawson L.D., Wang Z.J., Hughes B.G., Identification and HPLC quantitation of the sulfides and dialk(en)ylthiosulfinates in commercial garlic products, Planta Med., 57, pp. 363-370, (1991)

← 1 2 3 →