FLOW-INJECTION ANALYSIS OF NITRITE GENERATED BY NEUTROPHILS AND ENDOTHELIAL-CELLS

被引：6

作者：

HYBERTSON, BM ^{[1
]}

DUNHAM, AJ ^{[1
]}

TOMPSON, DC ^{[1
]}

TERADA, LS ^{[1
]}

REPINE, JE ^{[1
]}

机构：

[1] UNIV COLORADO,HLTH SCI CTR,SCH PHARM,DENVER,CO 80262

来源：

ANALYTICAL LETTERS | 1994年 / 27卷 / 15期

关键词：

NITRIC OXIDE; NITRITE; NEUTROPHILS; ENDOTHELIAL CELLS; CHEMILUMINESCENCE;

D O I：

10.1080/00032719408000312

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A flow injection method is described for the determination of low levels of nitrite ion in cell culture solutions. Nitrite-containing solutions are injected into a flowing stream containing phosphoric acid and iodide ion, which reduces nitrite to nitric oxide. The solution is directed through a microporous tube out of which nitric oxide gas diffuses for detection by chemiluminescence. The method has been applied to analysis of nitrite generated by cultured bovine pulmonary artery endothelial cells and human neutrophils.

引用

页码：3081 / 3093

页数：13

共 27 条

[1] HYPEROXIA DAMAGES CULTURED ENDOTHELIAL-CELLS CAUSING INCREASED NEUTROPHIL ADHERENCE [J].

BOWMAN, CM ;

BUTLER, EN ;

REPINE, JE .

AMERICAN REVIEW OF RESPIRATORY DISEASE, 1983, 128 (03) :469-472

[2] KINETICS OF NITRIC-OXIDE AND HYDROGEN-PEROXIDE PRODUCTION AND FORMATION OF PEROXYNITRITE DURING THE RESPIRATORY BURST OF HUMAN NEUTROPHILS [J].

CARRERAS, MC ;

PARGAMENT, GA ;

CATZ, SD ;

PODEROSO, JJ ;

BOVERIS, A .

FEBS LETTERS, 1994, 341 (01) :65-68

[3] NEUTROPHILS AND MONONUCLEAR-CELLS FROM PATIENTS WITH CHRONIC GRANULOMATOUS-DISEASE RELEASE NITRIC-OXIDE [J].

CONDINONETO, A ;

MUSCARA, MN ;

GRUMACH, AS ;

CARNEIROSAMPAIO, MMS ;

DENUCCI, G .

BRITISH JOURNAL OF CLINICAL PHARMACOLOGY, 1993, 35 (05) :485-490

[4] DETERMINATION OF NITRATE AND NITRITE AT THE PARTS PER BILLION LEVEL BY CHEMI-LUMINESCENCE [J].

COX, RD .

ANALYTICAL CHEMISTRY, 1980, 52 (02) :332-335

[5]

DUNHAM AJ, 1994, UNPUB

[6]

DUNHAM AJ, 1994, THESIS U COLORADO

[7]

FAINT RW, 1991, BRIT J HAEMATOL, V77, P539

[8] THE BIOLOGY OF NITROGEN-OXIDES IN THE AIRWAYS [J].

GASTON, B ;

DRAZEN, JM ;

LOSCALZO, J ;

STAMLER, JS .

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 1994, 149 (02) :538-551

[9] NITRIC-OXIDE PRODUCTION BY HUMAN PERIPHERAL-BLOOD POLYMORPHONUCLEAR LEUKOCYTES [J].

GOODE, HF ;

WEBSTER, NR ;

HOWDLE, PD ;

WALKER, BE .

CLINICAL SCIENCE, 1994, 86 (04) :411-415

[10] ANALYSIS OF NITRATE, NITRITE, AND [N-15]-LABELED NITRATE IN BIOLOGICAL-FLUIDS [J].

GREEN, LC ;

WAGNER, DA ;

GLOGOWSKI, J ;

SKIPPER, PL ;

WISHNOK, JS ;

TANNENBAUM, SR .

ANALYTICAL BIOCHEMISTRY, 1982, 126 (01) :131-138

← 1 2 3 →