CALCIUM-INDUCED CHANGES IN ANNEXIN-V BEHAVIOR IN SOLUTION AS SEEN BY PROTON NMR-SPECTROSCOPY

被引：13

作者：

NEUMANN, JM

SANSON, A

LEWITBENTLEY, A

机构：

[1] UNIV PARIS 06,SERV BIOPHYS,PARIS,FRANCE

[2] CTR UNIV PARIS SUD,UTILISAT RAYONNEMENT ELECTROMAGNET LAB,ORSAY,FRANCE

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1994年 / 225卷 / 03期

关键词：

D O I：

10.1111/j.1432-1033.1994.0819b.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The behaviour of human annexin V in the presence of calcium was studied by NMR. We observe the formation of well defined dimers, as well as a change in the local dynamics of one His side chain. We assign the observed changes to either His98 or His267 residues and conclude that they could be related either to the hinge-bending motion reported from crystal structures, or to a local side chain rearrangement within the calcium-binding loops concerned. Dimerization was also confirmed by a small-angle neutron-scattering experiment. Under the experimental conditions used, we do not observe the conformational change involving Trp187 seen in previous studies, which occurs at higher relative calcium concentrations.

引用

页码：819 / 825

页数：7

共 25 条

[1] AHN NG, 1988, J BIOL CHEM, V263, P18657
[2] STRUCTURE-FUNCTION ANALYSIS OF THE ION-CHANNEL SELECTIVITY FILTER IN HUMAN ANNEXIN-V
BERENDES, R
VOGES, D
DEMANGE, P
HUBER, R
BURGER, A
[J]. SCIENCE, 1993, 262 (5132) : 427 - 430
[3] STRUCTURE OF SOLUBLE AND MEMBRANE-BOUND HUMAN ANNEXIN-V
BRISSON, A
MOSSER, G
HUBER, R
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1991, 220 (02) : 199 - 203
[4] ASSIGNMENT OF HISTIDINE RESONANCES IN THE H-1-NMR (500 MHZ) SPECTRUM OF SUBTILISIN BPN' USING SITE-DIRECTED MUTAGENESIS
BYCROFT, M
FERSHT, AR
[J]. BIOCHEMISTRY, 1988, 27 (19) : 7390 - 7394
[5] PULSE METHODS FOR SIMPLIFICATION OF PROTEIN NMR-SPECTRA
CAMPBELL, ID
DOBSON, CM
WILLIAMS, RJP
WRIGHT, PE
[J]. FEBS LETTERS, 1975, 57 (01) : 96 - 99
[6] ANNEXIN-V FORMS CALCIUM-DEPENDENT TRIMERIC UNITS ON PHOSPHOLIPID-VESICLES
CONCHA, NO
HEAD, JF
KAETZEL, MA
DEDMAN, JR
SEATON, BA
[J]. FEBS LETTERS, 1992, 314 (02): : 159 - 162
[7] RAT ANNEXIN-V CRYSTAL-STRUCTURE - CA2+-INDUCED CONFORMATIONAL-CHANGES
CONCHA, NO
HEAD, JF
KAETZEL, MA
DEDMAN, JR
SEATON, BA
[J]. SCIENCE, 1993, 261 (5126) : 1321 - 1324
[8] CRYSTAL AND MOLECULAR-STRUCTURE OF HUMAN ANNEXIN-V AFTER REFINEMENT - IMPLICATIONS FOR STRUCTURE, MEMBRANE-BINDING AND ION CHANNEL FORMATION OF THE ANNEXIN FAMILY OF PROTEINS
HUBER, R
BERENDES, R
BURGER, A
SCHNEIDER, M
KARSHIKOV, A
LUECKE, H
ROMISCH, J
PAQUES, E
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1992, 223 (03) : 683 - 704
[9] THE CRYSTAL AND MOLECULAR-STRUCTURE OF HUMAN ANNEXIN-V, AN ANTICOAGULANT PROTEIN THAT BINDS TO CALCIUM AND MEMBRANES
HUBER, R
ROMISCH, J
PAQUES, EP
[J]. EMBO JOURNAL, 1990, 9 (12) : 3867 - 3874
[10] THE CALCIUM-BINDING SITES IN HUMAN ANNEXIN-V BY CRYSTAL-STRUCTURE ANALYSIS AT 2.0 A RESOLUTION - IMPLICATIONS FOR MEMBRANE-BINDING AND CALCIUM-CHANNEL ACTIVITY
HUBER, R
SCHNEIDER, M
MAYR, I
ROMISCH, J
PAQUES, EP
[J]. FEBS LETTERS, 1990, 275 (1-2) : 15 - 21

← 1 2 3 →