ELECTROPHORETIC SEPARATION OF LARGE PROTEOGLYCANS IN LARGE-PORE POLYACRYLAMIDE GRADIENT GELS (1.32-10.0-PERCENT-T) AND A ONE-STEP PROCEDURE FOR SIMULTANEOUS STAINING OF PROTEINS AND PROTEOGLYCANS

A procedure is described for the preparation of 1.32-10% polyacrylamide gradient gels. Loose polyacrylamide gel on the top side of the gradient was stabilized with a layer of 0.4% agarose gel which also formed sample wells. The upper limit of separation achieved in these gels was estimated to be approximately 2 × 106 using globular protein standards. However, large aggregating proteoglycans from cartilage which have a molecular weight range of 1-4 × 106 penetrate and separate in these gels. A simple one-step procedure is also described for simultaneous staining of proteins and large proteoglycans in polyacrylamide gels. © 1991.