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THE RIBOSOMAL-RNA-ENCODING DNA ARRAY HAS AN ALTERED STRUCTURE IN TOPOISOMERASE-I MUTANTS OF SACCHAROMYCES-CEREVISIAE

被引：38
作者：
CHRISTMAN, MF
DIETRICH, FS
LEVIN, NA
SADOFF, BU
FINK, GR
机构：
[1] WHITEHEAD INST BIOMED RES,9 CAMBRIDGE CTR,CAMBRIDGE,MA 02142
[2] MIT,DEPT BIOL,CAMBRIDGE,MA 02139
[3] UNIV CALIF SAN FRANCISCO,DEPT RADIAT ONCOL,SAN FRANCISCO,CA 94143
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1993年 / 90卷 / 16期
关键词：
PULSED-FIELD GEL; TOP1; DNA REPLICATION;
D O I：
10.1073/pnas.90.16.7637
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
All the chromosomes from isogenic TOP1 and top1 strains have similar mobility on pulsed-field gels except for chromosome XII, which fails to migrate into the gels in top1 mutants. Chromosome XII contains the tandem repeats of rRNA-encoding DNA (rDNA). When a segment of chromosome XII containing only rDNA is transferred to chromosome III by a recombination event, chromosome III fails to enter a pulsed-field gel in extracts from top1 strains, indicating that the aberrant migration of chromosome XII in top1 mutants is caused by the presence of rDNA. Failure of chromosome XII to migrate into a pulsed-field gel occurs only in preparations from exponentially growing top1 cultures and not in preparations from stationary-phase top1 cultures. rDNA from a top1 strain does enter the gel if it is cut with an enzyme (Pst I) that cuts the tandem rDNA array into single 9-kb repeat units, indicating that more than a single repeat unit is required to maintain the aberrant structure.
引用
收藏
页码：7637 / 7641
页数：5
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