IMMUNOCYTOCHEMICAL LOCALIZATION OF UBIQUITIN-ACTIVATING ENZYME IN THE CELL-NUCLEUS

被引：18

作者：

COOK, JC

CHOCK, PB

机构：

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 174卷 / 02期

关键词：

D O I：

10.1016/0006-291X(91)91454-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ubiquitin-activating enzyme, "E1", is the first enzyme in the pathway leading to formation of ubiquitin-protein conjugates. We present immunocytochemical evidence that Ubiquitin-activating enzyme is concentrated in the cell nucleus. This finding points to the nucleus as the major site of action of this enzyme. Since ubiquitin itself is not similarly compartmentalized, this result suggests a high level of ubiquitin conjugate formation in the nucleus with a rapid turnover of ubiquitin conjugates. © 1991.

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页码：564 / 571

页数：8

相关论文

共 36 条

[1] IDENTITY OF THE 19S PROSOME PARTICLE WITH THE LARGE MULTIFUNCTIONAL PROTEASE COMPLEX OF MAMMALIAN-CELLS (THE PROTEASOME) [J].

ARRIGO, AP ;

TANAKA, K ;

GOLDBERG, AL ;

WELCH, WJ .

NATURE, 1988, 331 (6152) :192-194

[2] UBIQUITIN IS A HEAT-SHOCK PROTEIN IN CHICKEN-EMBRYO FIBROBLASTS [J].

BOND, U ;

SCHLESINGER, MJ .

MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (05) :949-956

[3] THE CHICKEN UBIQUITIN GENE CONTAINS A HEAT-SHOCK PROMOTER AND EXPRESSES AN UNSTABLE MESSENGER-RNA IN HEAT-SHOCKED CELLS [J].

BOND, U ;

SCHLESINGER, MJ .

MOLECULAR AND CELLULAR BIOLOGY, 1986, 6 (12) :4602-4610

[4] MICROINJECTION OF UBIQUITIN - INTRACELLULAR-DISTRIBUTION AND METABOLISM IN HELA-CELLS MAINTAINED UNDER NORMAL PHYSIOLOGICAL CONDITIONS [J].

CARLSON, N ;

RECHSTEINER, M .

JOURNAL OF CELL BIOLOGY, 1987, 104 (03) :537-546

[5] MICROINJECTION OF UBIQUITIN - CHANGES IN PROTEIN-DEGRADATION IN HELA-CELLS SUBJECTED TO HEAT-SHOCK [J].

CARLSON, N ;

ROGERS, S ;

RECHSTEINER, M .

JOURNAL OF CELL BIOLOGY, 1987, 104 (03) :547-555

[6] ATP-DEPENDENT CONJUGATION OF RETICULOCYTE PROTEINS WITH THE POLYPEPTIDE REQUIRED FOR PROTEIN-DEGRADATION [J].

CIECHANOVER, A ;

HELLER, H ;

ELIAS, S ;

HAAS, AL ;

HERSHKO, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (03) :1365-1368

[7]

CIECHANOVER A, 1982, J BIOL CHEM, V257, P2537

[8] THE UBIQUITIN-MEDIATED PROTEOLYTIC PATHWAY AND MECHANISMS OF ENERGY-DEPENDENT INTRACELLULAR PROTEIN-DEGRADATION [J].

CIECHANOVER, A ;

FINLEY, D ;

VARSHAVSKY, A .

JOURNAL OF CELLULAR BIOCHEMISTRY, 1984, 24 (01) :27-53

[9]

COOK J, 1988, Biofactors, V1, P133

[10]

DRISCOLL J, 1990, J BIOL CHEM, V265, P4789

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