CLONING AND EXPRESSION OF THE HPAL RESTRICTION-MODIFICATION GENES

被引：10

作者：

ITO, H ^{[1
]}

SHIMATO, H ^{[1
]}

SADAOKA, A ^{[1
]}

KOTANI, H ^{[1
]}

KIMIZUKA, F ^{[1
]}

KATO, I ^{[1
]}

机构：

[1] TAKARA SHUZO CO LTD,BIOTECHNOL,RES LABS,OTSU,SHIGA 52021,JAPAN

来源：

NUCLEIC ACIDS RESEARCH | 1992年 / 20卷 / 04期

关键词：

D O I：

10.1093/nar/20.4.705

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The genes from Haemophilus parainfluenzae encoding the HpaI restriction-modification system were cloned and expressed in Escherichia coli. From the DNA sequence, we predicted the HpaI endonuclease (R.HpaI) to have 254 amino acid residues (M(r) 29,630) and the HpaI methyltransferase (M.HpaI) to have 314 amino acid residues (37,390). The R.HpaI and M-HpaI genes overlapped by 16 base pairs on the chromosomal DNA. The genes had the same orientation. The clone, named E. coli HB101-HPA2, overproduced R.HpaI. R.HpaI activity from the clone was 100-fold that from H. parainfluenzae. The amino acid sequence of M.HpaI was compared with those of other type II methyltransferases.

引用

页码：705 / 709

页数：5

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