CHROMOSOMES OF SEQUOIA SEMPERVIRENS - 8-HYDROXY-QUINOLONE-CASTOR OIL PRETREATMENT FOR IMPROVING PREPARATION

被引：8

作者：

FOZDAR, BS

LIBBY, WJ

机构：

[1] School of Forestry, University of California, Berkeley, CA

来源：

STAIN TECHNOLOGY | 1968年 / 43卷 / 02期

关键词：

D O I：

10.3109/10520296809115050

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Living root tips of coast redwood were pretreated for 6 hr at 10-14 C in a homogenized mixture of 0.003 M aqueous solution of about 5 ml of 8-hydroxyquinoline and a drop of castor oil. They were rinsed 2-3 times in Carnoy's alcohol-chloroform-acetic acid (3:2:1), and fixation allowed to continue in this fluid for 1 hr at 60 C. They were then hydrolysed in 1 N HC1 at 60 C for 45 min; washed with distilled water, and squashed in a drop of aceto-carmine or aceto-orcein to make a temporary slide, subsequently made permanent by a quick-freezing method. Our work to date seems to confirm 2n = 66 for S. sempervirens. © 1968 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：97 / +

页数：1

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