IMMOBILIZATION OF ALKALINE PROTEASE ON NYLON

被引:26
作者
CHELLAPANDIAN, M [1 ]
SASTRY, CA [1 ]
机构
[1] INDIAN INST TECHNOL,DEPT CHEM ENGN,MADRAS 600036,TAMIL NADU,INDIA
来源
BIOPROCESS ENGINEERING | 1994年 / 11卷 / 01期
关键词
D O I
10.1007/BF00369610
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The parameters involved in immobilization of alkaline protease on nylon using glutaraldehyde as coupling agent and the characteristics of the immobilized enzyme were investigated. Optimum temperature and pH of both free and immobilized enzyme for the degradation of protein was found. Immobilized enzyme showed better thermal stability than the free enzyme. The reusability and storage stability of the immobilized enzyme was also studied.
引用
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页码:17 / 21
页数:5
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