POSSIBLE INVOLVEMENT OF RETINOID-LIKE COFACTOR IN SERUM IN HEMIN/PROTOPORPHYRIN-IX-INDUCED DIFFERENTIATION OF HUMAN LEUKEMIA K562 CELLS

被引：10

作者：

NAKAJIMA, O

KAGECHIKA, H

SHUDO, K

HASHIMOTO, Y

IWASAKI, S

机构：

[1] UNIV TOKYO,INST MOLEC & CELLULAR BIOSCI,BUNKYO KU,TOKYO 113,JAPAN

[2] UNIV TOKYO,FAC PHARMACEUT SCI,BUNKYO KU,TOKYO 113,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 206卷 / 03期

关键词：

D O I：

10.1006/bbrc.1995.1142

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Serum is generally added to media used for mammalian cell culture and for investigation of in vitro cell differentiation induction. Erythroid differentiation of human leukemia cell line K562 is induced by hemin of protoporphyrin IX in the presence of fetal bovine serum (FBS). Addition to the medium of retinoids enhanced the differentiation, and addition of retinoid antagonists suppressed the differentiation. Making the added FBS retinoid-free, i.e., treatment of the serum with charcoal, also reduced the efficiency of the cell differentiation induced by hemin or protoporphyrin IX. These facts suggest that retinoids or retinoid-like cofactor(s) in serum are necessary for hemin/protoporphyrin-IX-induced erythroid differentiation of K562 cells. (C) 1995 Academic Press, Inc.

引用

收藏

页码：1003 / 1010

页数：8

相关论文

共 16 条

[1] A RETINOIC ACID RECEPTOR-ALPHA ANTAGONIST SELECTIVELY COUNTERACTS RETINOIC ACID EFFECTS [J].

APFEL, C ;

BAUER, F ;

CRETTAZ, M ;

FORNI, L ;

KAMBER, M ;

KAUFMANN, F ;

LEMOTTE, P ;

PIRSON, W ;

KLAUS, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (15) :7129-7133

[2] INHIBITION OF MOUSE GATA-1 FUNCTION BY THE GLUCOCORTICOID RECEPTOR - POSSIBLE MECHANISM OF STEROID INHIBITION OF ERYTHROLEUKEMIA CELL-DIFFERENTIATION [J].

CHANG, TJ ;

SCHER, BM ;

WAXMAN, S ;

SCHER, W .

MOLECULAR ENDOCRINOLOGY, 1993, 7 (04) :528-542

[3]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2

[4] RETINYL ACETATE AND ALL-TRANS-RETINOIC ACID ENHANCE ERYTHROID COLONY FORMATION INVITRO BY CIRCULATING HUMAN PROGENITORS IN AN IMPROVED SERUM-FREE MEDIUM [J].

CORREA, PN ;

AXELRAD, AA .

INTERNATIONAL JOURNAL OF CELL CLONING, 1992, 10 (05) :286-291

[5] RETINOIC ACID ENHANCES GROWTH OF HUMAN EARLY ERYTHROID PROGENITOR CELLS-INVITRO [J].

DOUER, D ;

KOEFFLER, HP .

JOURNAL OF CLINICAL INVESTIGATION, 1982, 69 (04) :1039-1041

[6] RETINOBENZOIC ACIDS .6. RETINOID ANTAGONISTS WITH A HETEROCYCLIC RING [J].

EYROLLES, L ;

KAGECHIKA, H ;

KAWACHI, E ;

FUKASAWA, H ;

IIJIMA, T ;

MATSUSHIMA, Y ;

HASHIMOTO, Y ;

SHUDO, K .

JOURNAL OF MEDICINAL CHEMISTRY, 1994, 37 (10) :1508-1517

[7]

Eyrolles L., 1992, MED CHEM RES, V2, P361

[8] EARLY PROTOONCOGENE EXPRESSION DURING HEMIN-INDUCED DIFFERENTIATION OF HUMAN ERYTHROLEUKEMIC CELLS [J].

GRIFFIN, MO ;

NELSON, JC ;

ABRAHAM, NG .

LEUKEMIA RESEARCH, 1990, 14 (11-12) :997-+

[9] EXPRESSION OF RETINOIC ACID RECEPTOR GENES AND THE LIGAND-BINDING SELECTIVITY OF RETINOIC ACID RECEPTORS (RARS) [J].

HASHIMOTO, Y ;

KAGECHIKA, H ;

SHUDO, K .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 166 (03) :1300-1307

[10] RETINOBENZOIC ACIDS .1. STRUCTURE-ACTIVITY-RELATIONSHIPS OF AROMATIC AMIDES WITH RETINOIDAL ACTIVITY [J].

KAGECHIKA, H ;

KAWACHI, E ;

HASHIMOTO, Y ;

HIMI, T ;

SHUDO, K .

JOURNAL OF MEDICINAL CHEMISTRY, 1988, 31 (11) :2182-2192