CLONING, SEQUENCE-ANALYSIS AND EXPRESSION OF 2 FORMS OF MESSENGER-RNA CODING FOR THE HUMAN BETA(2)-SUBUNIT OF THE GABA(A)-RECEPTOR

被引：29

作者：

MCKINLEY, DD ^{[1
]}

LENNON, DJ ^{[1
]}

CARTER, DB ^{[1
]}

机构：

[1] UPJOHN CO,CNS DIS RES 7251209721,KALAMAZOO,MI 49001

来源：

MOLECULAR BRAIN RESEARCH | 1995年 / 28卷 / 01期

关键词：

GABA(A) RECEPTOR SUBUNIT; CDNA; MESSENGER-RNA; POLYMERASE CHAIN REACTION; NORTHERN BLOT; SOUTHERN BLOT; SPLICE VARIANT; CALMODULIN-DEPENDENT PROTEIN KINASE II;

D O I：

10.1016/0169-328X(94)00228-7

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Two forms of cDNA coding for the human GABA(A) beta(2) subunit have been cloned and sequenced. The two sequences differ by a 114 base pair insertion. The insert contains a phosphorylation consensus sequence for calmodulin-dependent protein kinase II. Quantitative PCR studies show that h beta(2L) cDNA represents 10-15% of total h beta(2) cDNA in the 10 brain substructures tested. Analysis of human genomic southern blots suggests that the two forms might arise by differential splicing.

引用

页码：175 / 179

页数：5

共 12 条

[1]

ALMS WJ, 57TH ANN M AM COLL R

[2] SUPERCOIL SEQUENCING - A FAST AND SIMPLE METHOD FOR SEQUENCING PLASMID DNA [J].