PRIMARY STRUCTURE AND EXPRESSION OF A NUCLEAR-CODED SUBUNIT OF COMPLEX-I HOMOLOGOUS TO PROTEINS SPECIFIED BY THE CHLOROPLAST GENOME

被引：48

作者：

VIDEIRA, A ^{[1
]}

TROPSCHUG, M ^{[1
]}

WERNER, S ^{[1
]}

机构：

[1] UNIV MUNICH,INST PHYSIOL CHEM,W-8000 MUNICH 2,GERMANY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 171卷 / 03期

关键词：

D O I：

10.1016/0006-291X(90)90807-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 31-kDa subunit of complex I from Neurospora crassa, of nuclear origin, was cloned. The precursor polypeptide (33 kDa) could be efficiently expressed in an in vitro system for transcription and translation. The processing of the precursor to the mature protein was also obtained in vitro. An open reading frame coding for a precursor protein of 283 amino acids (32247 Da) was found by DNA sequencing. The predicted primary structure shows significant homology with proteins made in chloroplast. This supports the hypothesis that an enzyme similar to respiratory chain NADH dehydrogenase might exist in these organelles. © 1990.

引用

收藏

页码：1168 / 1174

页数：7

相关论文

共 23 条

[1] SEQUENCE AND ORGANIZATION OF THE HUMAN MITOCHONDRIAL GENOME [J].

ANDERSON, S ;

BANKIER, AT ;

BARRELL, BG ;

DEBRUIJN, MHL ;

COULSON, AR ;

DROUIN, J ;

EPERON, IC ;

NIERLICH, DP ;

ROE, BA ;

SANGER, F ;

SCHREIER, PH ;

SMITH, AJH ;

STADEN, R ;

YOUNG, IG .

NATURE, 1981, 290 (5806) :457-465

[2] EVIDENCE FOR A RESPIRATORY-CHAIN IN THE CHLOROPLAST [J].

BENNOUN, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (14) :4352-4356

[3] SCREENING GAMMAGT RECOMBINANT CLONES BY HYBRIDIZATION TO SINGLE PLAQUES INSITU [J].

BENTON, WD ;

DAVIS, RW .

SCIENCE, 1977, 196 (4286) :180-182

[4] URF6, LAST UNIDENTIFIED READING FRAME OF HUMAN MTDNA, CODES FOR AN NADH DEHYDROGENASE SUBUNIT [J].

CHOMYN, A ;

CLEETER, MWJ ;

RAGAN, CI ;

RILEY, M ;

DOOLITTLE, RF ;

ATTARDI, G .

SCIENCE, 1986, 234 (4776) :614-618

[5] 6 UNIDENTIFIED READING FRAMES OF HUMAN MITOCHONDRIAL-DNA ENCODE COMPONENTS OF THE RESPIRATORY-CHAIN NADH DEHYDROGENASE [J].

CHOMYN, A ;

MARIOTTINI, P ;

CLEETER, MWJ ;

RAGAN, CI ;

MATSUNOYAGI, A ;

HATEFI, Y ;

DOOLITTLE, RF ;

ATTARDI, G .

NATURE, 1985, 314 (6012) :592-597

[6]

CHOMYN A, 1988, J BIOL CHEM, V263, P16395

[7] A HOMOLOG OF THE NUCLEAR CODED 49 KD SUBUNIT OF BOVINE MITOCHONDRIAL NADH-UBIQUINONE REDUCTASE IS CODED IN CHLOROPLAST DNA [J].

FEARNLEY, IM ;

RUNSWICK, MJ ;

WALKER, JE .

EMBO JOURNAL, 1989, 8 (03) :665-672

[8] MITOCHONDRIAL PROTEIN IMPORT [J].

HARTL, FU ;

PFANNER, N ;

NICHOLSON, DW ;

NEUPERT, W .

BIOCHIMICA ET BIOPHYSICA ACTA, 1989, 988 (01) :1-45

[9] MITOCHONDRIAL PROTEIN IMPORT - IDENTIFICATION OF PROCESSING PEPTIDASE AND OF PEP, A PROCESSING ENHANCING PROTEIN [J].

HAWLITSCHEK, G ;

SCHNEIDER, H ;

SCHMIDT, B ;

TROPSCHUG, M ;

HARTL, FU ;

NEUPERT, W .

CELL, 1988, 53 (05) :795-806

[10] DELETIONS OF MUSCLE MITOCHONDRIAL-DNA IN PATIENTS WITH MITOCHONDRIAL MYOPATHIES [J].

HOLT, IJ ;

HARDING, AE ;

MORGANHUGHES, JA .

NATURE, 1988, 331 (6158) :717-719