MESSENGER-RNAS CODING FOR RECEPTORS AND CHANNELS IN THE CEREBRAL-CORTEX OF ADULT AND AGED RATS

被引:13
作者
CARPENTER, MK
PARKER, I
MILEDI, R
机构
[1] Laboratory of Cellular and Molecular Neurobiology, Department of Psychobiology, University of California, Irvine
来源
MOLECULAR BRAIN RESEARCH | 1992年 / 13卷 / 1-2期
关键词
AGING; MESSENGER RNA; OOCYTE; RECEPTOR; CHANNEL; CEREBRAL CORTEX;
D O I
10.1016/0169-328X(92)90038-D
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Poly(A)+ mRNAs from the cerebral cortex of aged (24 months) and young adult (3 months) rats were isolated and injected into Xenopus oocytes to express functional neurotransmitter receptors and voltage-operated channels. Electrophysiological recordings of induced membrane currents were used as a measure of the relative amounts of mRNA encoding different receptors and channels, and to study their functional properties. There were no large differences apparent between mRNAs from aged and adult rats, in marked contrast to the dramatic (1000-fold) changes in mRNA expression that occur during embryonic and postnatal development. The membrane currents induced by glutamate or acetylcholine (ACh) application were roughly one third smaller in oocytes injected with mRNA from aged cerebral cortex than in oocytes injected with mRNA from adult cerebral cortex, whereas currents induced by gamma-aminobutyric acid (GABA), kainate or serotonin (5-HT) application, and by activation of voltage-operated Na+ and Ca2+ channels were not significantly different. We did not observe any age-related differences in the properties of the receptors and channels studied.
引用
收藏
页码:1 / 5
页数:5
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