PURIFICATION, N-TERMINAL SEQUENCE AND STRUCTURAL CHARACTERIZATION OF A TOXIC PROTEIN FROM THE INDIAN SCORPION-VENOM BUTHUS-TAMULUS

被引：6

作者：

LALA, K ^{[1
]}

NARAYANAN, P ^{[1
]}

机构：

[1] UNIV BOMBAY,DEPT LIFE SCI,BOMBAY 400098,INDIA

来源：

TOXICON | 1994年 / 32卷 / 03期

关键词：

D O I：

10.1016/0041-0101(94)90085-X

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

A toxic protein component (Bt-II) was isolated from the venom of the Indian scorpion, Buthus tamulus, by ion-exchange chromatography on Biorex-70 and reverse phase high pressure liquid chromatography (RP-HPLC). This 7800 mol. wt protein was found to have an LD50 value of 2.25 mg/kg body weight in mice by subcutaneous injection. Amino acid composition of the protein revealed the presence of aromatic and basic amino acids; the N-terminal amino acid sequence was also determined. Structural characterization of Bt-II, based on circular dichroic and fluorescent quenching studies, has also been reported.

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页码：325 / 338

页数：14

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