CAPILLARY ELECTROPHORESIS FOURIER-TRANSFORM ION-CYCLOTRON RESONANCE MASS-SPECTROMETRY WITH SUSTAINED OFF-RESONANCE IRRADIATION FOR THE CHARACTERIZATION OF PROTEIN AND PEPTIDE MIXTURES

被引：42

作者：

HOFSTADLER, SA ^{[1
]}

WAHL, JH ^{[1
]}

BAKHTIAR, R ^{[1
]}

ANDERSON, GA ^{[1
]}

BRUCE, JE ^{[1
]}

SMITH, RD ^{[1
]}

机构：

[1] PACIFIC NW LAB, DEPT CHEM SCI, CHEM METHODS & SEPARAT GRP, P8-19, RICHLAND, WA 99352 USA

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 1994年 / 5卷 / 10期

关键词：

D O I：

10.1016/1044-0305(94)87014-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new approach to protein and peptide analysis that involves the coupling of on-line capillary electrophoresis-electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry with a variation of sustained off-resonance irradiation is described. With this technique, multiple irradiation frequencies are broadcast simultaneously, which yields fragmentation of species at different mass-to-charge ratio values from the same waveform. In conjunction with capillary electrophoresis, this technique can provide sequence information from small amounts of proteins or peptides in complex mixtures. Initial results obtained from a mixture of gramicidin S (1141 u), bee venom melittin (2845 u), and equine apomyoglobin (16,951 u) are presented.

引用

页码：894 / 899

页数：6

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