ADSORPTION AND DESORPTION STUDIES OF POLYADENYLIC-ACID ONTO POSITIVELY CHARGED LATEX-PARTICLES

被引：32

作者：

ELAISSARI, A ^{[1
]}

PICHOT, C ^{[1
]}

DELAIR, T ^{[1
]}

CROS, P ^{[1
]}

KURFURST, R ^{[1
]}

机构：

[1] ECOLE NORMALE SUPER LYON,BIOMERIEUX,SONDES NUCL LAB,CNRS,UNITE MIXTE,F-69364 LYON 07,FRANCE

来源：

LANGMUIR | 1995年 / 11卷 / 04期

关键词：

D O I：

10.1021/la00004a036

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The adsorption of single-stranded polyadenylic acid (poly dA) (30 bases) onto cationic polystyrene latex particles was studied. The latex particles were prepared by soap-free emulsion copolymerization of styrene and vinyl benzylamine hydrochloride. Adsorption experiments were carried out using a fluorescein-labeled polyadenylic acid and adsorption isotherms were established for constant ionic strength in a pH range of 4-9. Poly dA adsorption was optimal at low pH and decreased upon increasing pH. Desorption of oligodeoxyribonucleotides was studied by washing the latex particles and by changing the pH. The adsorption of polyadenylic acid onto cationic latexes is interpreted based upon the contributions of hydrophobic and electrostatic interactions.

引用

页码：1261 / 1267

页数：7

共 27 条

[1] ALBERTSEN C, 1990, ANAL BIOCHEM, V189, P40
[2] ANDRADE JD, 1985, ADSORPTION SURFACE I, V2
[3] BLACKBURN GM, 1990, NUCLEIC ACIDS CHEM B
[4] CANTOR CR, 1980, BIOPHYSICAL CHEM, V3
[5] CARUTHERS MH, 1992, METHOD ENZYMOL, V211, P3
[6] IMMOBILIZATION OF POLYNUCLEOTIDES ON MAGNETIC PARTICLES - FACTORS INFLUENCING HYBRIDIZATION EFFICIENCY
DAY, PJR
FLORA, PS
FOX, JE
WALKER, MR
[J]. BIOCHEMICAL JOURNAL, 1991, 278 : 735 - 740
[7] DELAIR T, Patent No. 9106068
[8] DELAIR T, 1994, COLLOID POLYM SCI, P342
[9] DELAIR T, 1993, COLLOID POLYM SCI, P271
[10] ADSORPTION OF OLIGONUCLEOTIDES ONTO NEGATIVELY AND POSITIVELY CHARGED LATEX-PARTICLES
ELAISSARI, A
CROS, P
PICHOT, C
LAURENT, V
MANDRAND, B
[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 1994, 83 (01) : 25 - 31

← 1 2 3 →