RELIEF OF CYTOTOXICITY AND ENHANCEMENT OF INTERFERON INDUCER ACTIVITY OF DOUBLE-STRANDED-RNA BY EUKARYOTIC INITIATION FACTOR-II

被引：7

作者：

HARARY, R ^{[1
]}

GONSKY, R ^{[1
]}

ITAMAR, D ^{[1
]}

KAEMPFER, R ^{[1
]}

机构：

[1] HEBREW UNIV JERUSALEM,HADASSAH MED SCH,DEPT MOLEC VIROL,IL-91010 JERUSALEM,ISRAEL

来源：

VIROLOGY | 1990年 / 174卷 / 02期

关键词：

D O I：

10.1016/0042-6822(90)90103-X

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Double-stranded RNA (dsRNA) is a powerful interferon inducer but also possesses toxic properties. dsRNA was shown previously to inhibit translation by causing the inactivation of eukaryotic initiation factor 2 (eIF-2) and to bind tightly to this protein. The cytoxicity of dsRNA was analyzed simultaneously with the induction of interferon in murine fibroblast cultures. Incubation of dsRNA with eIF-2 leads to a significant reduction in toxicity, concomitant with a marked stimulation of interferon induction. The enhancement of dsRNA-dependent interferon induction by eIF-2 is sensitive to a monoclonal antibody directed against the β of eIF-2. When injected in combination with dsRNA, elF-2 potentiates the dsRNA-dependent survival of mice infected with a lethal dose of Mango virus. © 1990.

引用

页码：494 / 503

页数：10

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