PROTEIN-KINASE-C ACTIVITY IN RAT-BRAIN CORTEX

被引：9

作者：

CRUMRINE, RC ^{[1
]}

LAMANNA, JC ^{[1
]}

机构：

[1] CASE WESTERN RESERVE UNIV,DEPT PHYSIOL & BIOPHYS,CLEVELAND,OH 44106

来源：

JOURNAL OF NEUROCHEMISTRY | 1990年 / 55卷 / 03期

关键词：

Activation; In situ fixation; Ischemia; Protein kinase C;

D O I：

10.1111/j.1471-4159.1990.tb04566.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Abstract: The procedure used to obtain cerebral tissue for analysis of protein kinase C (PKC) activity may affect the subcellular distribution of the enzyme. We compared different methods of tissue preparation and found that the proportion of PKC activity associated with the paniculate fraction of the cerebral cortex was only 30% when the brain was frozen in situ while the animal was on life support or after decapitation followed by delayed freezing. Other methods of obtaining cerebral tissue resulted in 49–56% of the PKC activity in the particulate fraction. Freezing per se had no apparent effect on the activity or subcellular distribution of PKC. In addition, whenever the particulate PKC activity was high (>48%), there was also a significant increase in the proportion of particulate protein (from 51 to ∼63%, p > 0.05). Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：826 / 831

页数：6

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