THE USE OF A HAPTEN-FAB CONJUGATE TO SENSITIZE TARGET-CELLS FOR ANTIBODY-DEPENDENT COMPLEMENT-MEDIATED LYSIS AND ANTIBODY-DEPENDENT CELL-MEDIATED CYTOTOXICITY

被引：13

作者：

AASE, A

MICHAELSEN, TE

机构：

[1] Department of Immunology, National Institute of Public Health, Oslo

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1991年 / 136卷 / 02期

关键词：

HAPTEN-FAB' CONJUGATE; ANTIBODY-DEPENDENT CELL-MEDIATED CYTOTOXICITY; ANTIBODY-DEPENDENT COMPLEMENT-MEDIATED LYSIS; (ANTIBODY);

D O I：

10.1016/0022-1759(91)90005-Z

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

NIP-conjugated Fab' fragments from a rabbit hyperimmunized with sheep red blood cells (SRBC) were used to link the hapten NIP to target cells for antibody-dependent cell-mediated cytotoxicity (ADCC) and antibody-dependent complement-mediated lysis (ADCML). Target cells (SRBC) labelled with this NIP-Fab' complex were compared with SRBC directly haptenized with NIP in ADCC and ADCML assay using a NIP specific IgGl chimeric antibody. Both methods yielded almost identical results. Using the NIP-Fab' conjugate identical target cell haptenization was readily achieved from experiment to experiment. Using conjugates of different NIP/Fab' ratios it should be possible to study how such changes influence antibody effector functions.

引用

页码：185 / 191

页数：7

共 9 条

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