ADAPTATION OF PSEUDOMONAS-AERUGINOSA TO 2,2'-METHYLENEBIS (4-CHLOROPHENOL)

被引:11
作者
BROZEL, VS
CLOETE, TE
机构
[1] Environmental Biotechnology Laboratory, Department of Microbiology and Plant Pathology, University of Pretoria. Pretoria
来源
JOURNAL OF APPLIED BACTERIOLOGY | 1993年 / 74卷 / 01期
关键词
D O I
10.1111/j.1365-2672.1993.tb03001.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A Culture of Pseudomonas aeruginosa, isolated from a cooling water system, was grown in the presence of sub-inhibitory concentrations of 2,2'-methylenebis(4-chlorophenol) (MBC). It adapted to increasing concentrations from an initial minimum inhibitory concentration of 36 mug ml-1 to the highest, 80 mug ml-1. Resistant cultures exhibited a higher survival rate when exposed to 320 mug ml-1 than did the original strain. Lipopolysaccharide and outer membrane protein profiles were determined by SDS PAGE. No changes were detected in lipopolysaccharide profiles. The quantity of OprP, the phosphate uptake protein in the outer membrane, decreased to a low level correlating with decreased phosphate (P(i)) uptake during growth. It is proposed that OprP is the place of entry for MBC and that the cell can adapt by decreasing the level of OprP in the outer membrane.
引用
收藏
页码:94 / 99
页数:6
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