QUANTITATIVE ASPECTS OF INDIRECT UV DETECTION IN CAPILLARY ZONE ELECTROPHORESIS

被引:108
作者
NIELEN, MWF
机构
[1] AKZO Research Laboratories Arnhem, Corporate Research, Analytical Chemistry Department, 6800 SB Arnhem
来源
JOURNAL OF CHROMATOGRAPHY | 1991年 / 588卷 / 1-2期
关键词
D O I
10.1016/0021-9673(91)85040-M
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Quantitative aspects of indirect UV absorbance detection in capillary zone electrophoresis were studied using the separation of sodium alkylsulphate surfactants as a model system. Nearly uniform response factors, excellent reproducibility of electrophoretic mobilities and linearity of the detection signal can be obtained when veronal buffer is used as the UV-absorbing background electrolyte. The feasibility of the system for the analysis of commercial mixtures of primary sodium alkyl sulphates is demonstrated.
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页码:321 / 326
页数:6
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