PARAFFIN EMBEDDED BREAST CARCINOMAS FOR THE IMMUNOHISTOCHEMICAL STUDY OF PROGNOSTIC FACTORS

被引：10

作者：

ELIAS, JM

QIAO, L

HEIMANN, A

ENGELLENNER, W

ABEL, W

机构：

[1] Department of Pathology, University Hospital, Stony Brook, NY

[2] Henan Medical University, University Hospital, Henan

[3] Department of Surgery, University Hospital, Stony Brook, NY

来源：

JOURNAL OF HISTOTECHNOLOGY | 1992年 / 15卷 / 04期

基金：

美国国家卫生研究院;

关键词：

BREAST CANCER; IMMUNOHISTOCHEMISTRY; PARAFFIN SECTIONS; PROGNOSIS;

D O I：

10.1179/his.1992.15.4.315

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

There is increasing evidence that prognostic markers, including steroid receptors (estrogen and progesterone), estrogen-regulated proteins (pS2 and cathepsin-D), proliferation markers (proliferating cell nuclear antigen), and growth factors (epidermal growth factor receptor) can be useful in the management of patients with breast cancer. However, a full panel of assays would need a large sample of biopsy tissue, and increasing public awareness of the need for early detection has resulted in a continuous decrease in size of individual biopsies. Commercial antibodies that perform on routine paraffin sections have now made it practical to perform an array of immunohistochemical assays on paraffin embedded tissue. Our experience with the routinely processed biopsies of 27 patients provides convincing evidence of the feasibility of determining some of the more widely used prognostic markers in breast cancer from a single paraffin block.

引用

页码：315 / 320

页数：6

共 49 条

[1]

Silverberg E., Lubera J., Cancer statistics, Cancer, 37, (1987)

[2]

Paulsen H.E., In vitro tests and hormonal treatment of breast cancer, Progress in Surgical Pathology, pp. 37-65, (1983)

[3]

Mc Carty K.S., Miller L.S., Cox E.B., Et al., Estrogen receptor analysis: Correlation of biochemical and immunohistochemical methods using monoclonal anti-receptor antibodies, Arch Pathol Lab Med, 109, (1985)

[4]

Raymond W.A., Leong A.S., Oestrogen receptor staining of paraffin-embedded breast carcinomas following short fixation in formalin: A comparison with cytosolic and frozen section receptor analysis, J Pathol, 160, pp. 295-303, (1990)

[5]

Elias J.M., Heimann A., Carn T., Et al., Estrogen receptor localization in paraffin sections by enzyme digestion, repeated applications of primary antibody and imidazole, J Histotechnol, 13, pp. 29-33, (1990)

[6]

Andersen J., Thorpe S.M., King W.J., Et al., The prognostic value of immunohistochemical estrogen receptor analysis in paraffin-embedded and frozen sections versus that of steroid binding assays, Eurj Cancer, 26, pp. 442-449, (1990)

[7]

Epidermal growth factor in breast cancer, Int J Biochem, 22, pp. 939-945, (1990)

[8]

Rochefort H., Cathepsin D in breast cancer. Plenary lecture- 12th San Antonio Breast Cancer Symposium, Breast Cancer Res Treat, 16, pp. 3-13, (1990)

[9]

Vacca L.L., Abrahams S.J., Naftchi N.E., A modified peroxidaseantiperoxidase procedure for improved localization of tissue antigens. Localization of substance P in rat spinal cord, J Histochem Cytochem, 28, pp. 297-301, (1980)

[10]

Paradiso A., Tommasi S., Mangia A., Et al., Tumor-proliferative activity, progesterone receptor status, estrogen receptor breed, and clinical outcome of estrogen-positive advanced breast cancer, Cancer Res, 50, pp. 2958-2962, (1990)

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