REGULATION OF GLYCOLIPID SYNTHESIS IN HL-60 CELLS BY ANTISENSE OLIGODEOXYNUCLEOTIDES TO GLYCOSYLTRANSFERASE SEQUENCES - EFFECT ON CELLULAR-DIFFERENTIATION

被引：53

作者：

ZENG, GC ^{[1
]}

ARIGA, T ^{[1
]}

GU, XB ^{[1
]}

YU, RK ^{[1
]}

机构：

[1] VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,DEPT BIOCHEM & MOLEC BIOPHYS,RICHMOND,VA 23298

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1995年 / 92卷 / 19期

关键词：

GANGLIOSIDES; GENE EXPRESSION; CELL MATURATION;

D O I：

10.1073/pnas.92.19.8670

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Treatment of the human promyelocytic leukemia cell line HL-60 with antisense oligodeoxynucleotides to UDP-N-acetylgalactosamine:beta-1,4-N-acetyl transferase (GM2-synthase; EC 2.4.1.92) and CMP-sialic acid:alpha-2,8-sialyltransferase (GD3-synthase; EC 2.4.99.8) sequences effectively dean-regulated the synthesis of more complex gangliosides in the ganglioside synthetic pathways after GM3, resulting in a remarkable increase in endogenous GM3 with concomitant decreases in more complex gangliosides, The treated cells underwent monocytic differentiation as judged by morphological changes, adherent ability, and nitroblue tetrazolium staining, These data provide evidence that the increased endogenous ganglioside GM3 may play an important role in regulating cellular differentiation and that the antisense DNA technique proves to be a powerful tool in manipulating glycolipid synthesis in the cell.

引用

页码：8670 / 8674

页数：5

共 30 条

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