QUANTITATIVE REPRODUCIBILITY STUDY WITH AUTOMATED MICROCOLUMN LIQUID-CHROMATOGRAPHY

Studies were conducted to evaluate the quantitative reproducibility and long-term system stability for a reversed-phase microcolumn liquid chromatographic system. The relative standard deviation found was 0.38% for a major component and 0.7-1.6% for minor impurities using external standard calibration and automated sample injection. System longevity was studied over an eight-month operating period using repetitive injections of synthetic samples in an automated fashion. Excellent column stability was observed with minimal increase in operating pressure or observable loss in resolution. The results obtained suggest that microcolumn liquid chromatography is well-suited for routine applications.