PEPTIDE MIMICRY OF THE MENINGOCOCCAL GROUP-C CAPSULAR POLYSACCHARIDE

被引：116

作者：

WESTERINK, MAJ

GIARDINA, PC

APICELLA, MA

KIEBEREMMONS, T

机构：

[1] UNIV OKLAHOMA,DEPT MICROBIOL,OKLAHOMA CITY,OK 73190

[2] UNIV IOWA,DEPT MICROBIOL,IOWA CITY,IA 52242

[3] UNIV PENN,DEPT MED,PHILADELPHIA,PA 19104

[4] WISTAR INST ANAT & BIOL,PHILADELPHIA,PA 19104

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1995年 / 92卷 / 09期

关键词：

D O I：

10.1073/pnas.92.9.4021

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Sequence analysis of the variable regions of the heavy and light chains of the anti-idiotypic antibody 6F9, which mimics the meningococcal group C capsular polysaccharide (MCP), was performed. The immunogenic site on 6F9 responsible for inducing an anti-MCP antibody response was determined by means of sequence and computer model analysis of these data. Complementarity-determining region 3 (CDR3) was found to be unique in that the sequence tract YRY was exposed on the surface. A synthetic peptide spanning the CDR3 domain was synthesized and complexed to proteosomes (meningococcal group B outer membrane proteins), Immunizations of BALB/c mice with the peptide-proteosome complex resulted in a significant anti-MCP antibody response. Immunized mice were protected against infection with a lethal dose of Neisseria mellingitidis serogroup C.

引用

页码：4021 / 4025

页数：5

共 52 条

[1]

Tunkel A.R., Scheid W.M., Clin. Microbiol Rev., 6, pp. 118-136, (1993)

[2]

Werlin S.L., Am. J. Med. Genet, 46, pp. 455-456, (1993)

[3]

Friedland I.R., Klugman K.P., Am. J. Dis. Child., 146, pp. 920-923, (1992)

[4]

Andreoni J., Kayhty H., Densen P., J. Infect. Dis., 168, pp. 227-231, (1993)

[5]

Goldblatt D., Levinsky R.J., Turner M.W., J. Infect. Dis., 166, pp. 632-634, (1992)

[6]

Loughlin A.M., Marchant C.D., Lett S., Shapiro E.D., Pediatr. Infect. Dis. J., 11, pp. 374-379, (1992)

[7]

Gold R., Lepow M.L., J. Clin. Invest., 56, pp. 1536-1547, (1975)

[8]

Makela P.H., Peltola H., Kayhty H., Jousimies H., Pettay O., Ruoslahti E., Sivonen A., Renkonen O.V., J. Infect. Dis., 136, (1977)

[9]

Eskola J., Takala A.K., Kayhty H., Curr. Opin. Pediatr., 5, pp. 55-59, (1993)

[10]

Schneerson R., Levi L., Robbins J.B., Bryla D.M., Schiffman G., Lagergard T., Infect. Immun., 60, pp. 3528-3532, (1992)

← 1 2 3 4 5 6 →