ISOELECTRIC PROTEIN-PURIFICATION IN SEGMENTED IMMOBILIZED PH GRADIENTS - EFFECT OF SALTS ON RATE OF CONTAMINANTS REMOVAL

被引：16

作者：

BARZAGHI, B

RIGHETTI, PG

FAUPEL, M

机构：

[1] UNIV MILAN, FAC PHARM, CHAIR BIOCHEM, VIA CELORIA 2, I-20133 MILANO, ITALY

[2] UNIV MILAN, DEPT BIOMED SCI & TECHNOL, I-20133 MILANO, ITALY

[3] CIBA GEIGY AG, DIV PHARMACEUT, CHEM & BIOL RES LABS, CH-4002 BASEL, SWITZERLAND

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1987年 / 15卷 / 3-4期

关键词：

D O I：

10.1016/0165-022X(87)90116-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：177 / 187

页数：11

共 13 条

[1] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[2] ISOELECTRIC PROTEIN-PURIFICATION BY ORTHOGONALLY COUPLED HYDRAULIC AND ELECTRIC TRANSPORTS IN A SEGMENTED IMMOBILIZED PH GRADIENT
FAUPEL, M
BARZAGHI, B
GELFI, C
RIGHETTI, PG
[J]. JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 1987, 15 (3-4): : 147 - 161
[3] DIFFUSION-COEFFICIENTS OF PROTEINS IN CARRIER AMPHOLYTE VERSUS IMMOBILINE GELS
GELFI, C
BOSSI, ML
RIGHETTI, PG
[J]. JOURNAL OF CHROMATOGRAPHY, 1987, 390 (01): : 225 - 236
[4] ISOELECTRIC FOCUSING IN FREE AMPHOLINE SOLUTION AND ATTEMPTS AT ISOELECTRIC FOCUSING IN PH GRADIENTS CREATED IN ORDINARY BUFFERS
LUNDAHL
HJERTEN, S
[J]. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1973, 209 (JUN15) : 94 - 111
[5] RIGHETTI PG, 1984, J CHROMATOGR, V291, P31
[6] RIGHETTI PG, 1987, METHOD BIOCHEM ANAL, V32, P215
[7] RIGHETTI PG, 1987, J BIOCHEM BIOPH METH, V15, P163
[8] RIGHETTI PG, 1984, J CHROMATOGR, V300, P165
[9] RIGHETTI PG, 1986, GEL ELECTROPHORESIS, P141
[10] RIGHETTI PG, 1983, ISOELECTRIC FOCUSING, P204