DETERMINATION OF AMPICILLIN IN BIOLOGICAL-FLUIDS BY COUPLED-COLUMN LIQUID-CHROMATOGRAPHY AND POSTCOLUMN DERIVATIZATION

被引：12

作者：

LANBECKVALLEN, K

CARLQVIST, J

NORDGREN, T

机构：

[1] Department of Bioanalysis, Research and Development Laboratories, Astra Research Centre AB

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 567卷 / 01期

关键词：

D O I：

10.1016/0378-4347(91)80316-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A rapid and sensitive high-performance liquid chromatographic method has been developed for the determination of ampicillin in plasma and urine. The method involves a simple deproteinization step and separation on a coupled-column chromatographic system followed by post-column derivatization and fluorescence detection. The method has been used for the determination of ampicillin in various clinical studies. The high sensitivity makes it especially useful for small volumes, e.g. samples from pediatric patients.

引用

页码：121 / 128

页数：8

共 11 条

[1] AUTOMATED-DETERMINATION OF AMOXYCILLIN IN BIOLOGICAL-FLUIDS BY COLUMN SWITCHING IN ION-PAIR REVERSED-PHASE LIQUID-CHROMATOGRAPHIC SYSTEMS WITH POST-COLUMN DERIVATIZATION
CARLQVIST, J
WESTERLUND, D
[J]. JOURNAL OF CHROMATOGRAPHY, 1985, 344 (NOV): : 285 - 296
[2] REACTION DETECTOR WITH 3-DIMENSIONAL COILED OPEN TUBES IN HPLC
ENGELHARDT, H
NEUE, UD
[J]. CHROMATOGRAPHIA, 1982, 15 (07) : 403 - 408
[3] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY OF AMPICILLIN, AMOXICILLIN AND CICLACILLIN IN SERUM AND URINE USING A PRE-COLUMN REACTION WITH 1,2,4-TRIAZOLE AND MERCURY(II) CHLORIDE
HAGINAKA, J
WAKAI, J
[J]. ANALYST, 1985, 110 (11) : 1277 - 1281
[4] LIQUID-CHROMATOGRAPHIC DETERMINATION OF AMPICILLIN AND ITS METABOLITES IN HUMAN-URINE BY POSTCOLUMN ALKALINE-DEGRADATION
HAGINAKA, J
WAKAI, J
[J]. JOURNAL OF PHARMACY AND PHARMACOLOGY, 1987, 39 (01) : 5 - 8
[5] SIMULTANEOUS DETERMINATION OF AMPICILLIN AND SULBACTAM BY LIQUID-CHROMATOGRAPHY - POSTCOLUMN REACTION WITH SODIUM-HYDROXIDE AND SODIUM-HYPOCHLORITE USING AN ACTIVE HOLLOW-FIBER MEMBRANE REACTOR
HAGINAKA, J
NISHIMURA, Y
[J]. JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1990, 532 (01): : 87 - 94
[6] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF AMPICILLIN AND ITS METABOLITES IN RAT PLASMA, BILE AND URINE BY POSTCOLUMN DEGRADATION WITH SODIUM-HYPOCHLORITE
HAGINAKA, J
WAKAI, J
YASUDA, H
UNO, T
TAKAHASHI, K
KATAGI, T
[J]. JOURNAL OF CHROMATOGRAPHY, 1987, 400 : 101 - 111
[7] DETERMINATION OF AMPICILLIN, AMOXICILLIN, CEPHALEXIN, AND CEPHRADINE IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING FLUOROMETRIC DETECTION
MIYAZAKI, K
OHTANI, K
SUNADA, K
ARITA, T
[J]. JOURNAL OF CHROMATOGRAPHY, 1983, 276 (02): : 478 - 482
[8] LIQUID-CHROMATOGRAPHIC ASSAYS OF ANTIMICROBIAL AGENTS
RISTUCCIA, PA
[J]. JOURNAL OF LIQUID CHROMATOGRAPHY, 1987, 10 (2-3): : 241 - 276
[9] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY OF AMPICILLIN IN PLASMA AND BRONCHO-ALVEOLAR LAVAGE FLUID, USING FLUORESCENCE DETECTION
ROSSEEL, MT
BOGAERT, MG
VALCKE, YJ
[J]. CHROMATOGRAPHIA, 1989, 27 (5-6) : 243 - 246
[10] ANTIBIOTIC MONITORING IN BODY-FLUIDS
ROUAN, MC
[J]. JOURNAL OF CHROMATOGRAPHY, 1985, 340 (MAY): : 361 - 400

← 1 2 →