PROLIFERATIVE RESPONSE OF B-CHRONIC LYMPHOCYTIC-LEUKEMIA LYMPHOCYTES STIMULATED WITH IL2 AND SOLUBLE CD23

被引：8

作者：

BRIZARD, A

MOREL, F

LECRON, JC

DREYFUS, B

BRIZARD, F

BARRA, A

PREUDHOMME, JL

机构：

[1] Département d'Hématologie et Oncologic Médicale

[2] Laboratoire d'Immunologie et Interactions Moléculaires (CNRS URA 1172), CHU La Mitétrie, 86021 Poitiers Cedex

来源：

LEUKEMIA & LYMPHOMA | 1994年 / 14卷 / 3-4期

关键词：

B-CLL; SCD23; IL-2; PROLIFERATION; LYMPHOKINES;

D O I：

10.3109/10428199409049683

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The in vitro proliferative response of purified B-chronic lymphocytic leukemia (B-CLL) lymphocytes cultured in the presence of soluble CD23 (sCD23) with or without IL2 was compared to the responses induced by phorbol 12-myristate 13-acetate (PMA), Staphylococcus aureus strain Cowan I (SAC), IL1, IL2, IL4, IL6 and the combination of IL2 and interferon (IFN) cu or IFN gamma. As expected, B-CLL lymphocytes proliferated with PMA, SAC and IL2 with a clear enhancement of the IL2-induced response by IFN alpha or IFN gamma. They failed to proliferate in response to sCD23, IL1, IL4 or IL6 alone nor to the combinations of sCD23 and any of the 3 latter cytokines. However, sCD23 significantly increased the proliferation of B-CLL cells induced by IL2, suggesting a protective effect of sCD23 on apoptosis. Serum levels of sCD23 and CD23 membrane expression were high in every patient which is compatible with the hypothesis of an autocrine or paracrine activation loop. Detectable CD23 expression was lost in all cultures except for that stimulated by PMA. Only supernatants of PMA-stimulated cultures contained high sCD23 levels.

引用

页码：311 / 318

页数：8

共 37 条

[1] Bennett J.M., Catovsky D., Daniel M.T., Flandrin G., Galton D.A.G., Gralnick H.R., Sultan C., The French-American-British (FAB) Cooperative group. Proposals for the classification of chronic (mature) B and T lymphoid leukaemias, J. Clin. Pathol., 42, pp. 567-584, (1989)
[2] Delespesse G., Suter U., Mossalayi M.D., Bettler B., Sarfati M., Hofstetter H., Kilcherr E., Debre P., Dalloul A.H., Expression, structure and function of CD23 antigen, Adv. Immunol., 49, pp. 149-221, (1991)
[3] Kawabe T., Maekawa N., Maeda Y., Hosoda M., Yodoi J., Induction of FcRII/CD23 on phytohemagglutinin-activated human peripheral blood T lymphocytes. I. Enhancement by IL-2 and IL-4, J. Immunol., 147, pp. 548-553, (1991)
[4] Mossalayi M.D., Lecron J.C., Dalloul A.H., Sarfati M., Bertho J.M., Hofstetter H., Delespesse G., Debr S.P., Soluble CD23 (FceR11) and IL1 synergistically induce early human thymocyte maturation, J. Exp. Med., 171, pp. 959-964, (1990)
[5] Mossalayi M.D., Arock M., Bertho J.M., Blanc C., Dalloul A.H., Hofstetter H., Sarfati M., Delespesse G., Debre P., Proliferation of early human myeloid precursors induced by interleukin-1 and recombinant soluble CD23, Blood, 75, pp. 1924-1927, (1990)
[6] Liu Y.J., Cairns J.A., Holder M.J., Abbot S.D., Jansen K.U., Bonnefoy J.Y., Gordon J., MacLennan I.C.M., Recombinant 25-kDa CD23 and interleukin la promote the survival of germinal center B cells: evidence for bifurcation in the development of centrocytes rescued from apoptosis, Eur. J. Immunol., 21, pp. 1107-1114, (1991)
[7] Morel F., Goube de Laforest P., Brizard A., Gombert J., Lecron J.C., Identification of the B cell derived T cell colony promoting activity to soluble CD23, Eur. Cytokine. Netw., 3, pp. 485-494, (1992)
[8] Liu Y.J., Mason D.Y., Johnson G.D., Abbot S., Gregory C.D., Hardie D.L., Gordon J., MacLennan I.C.M., Germinal center cells express bcl-2 protein after activation by signals which prevent their entry into apoptosis, Eur. J. Immunol., 21, pp. 1905-1910, (1991)
[9] Sarfati M., Fournier S., Christoffersen M., Biron G., Expression of CD23 antigen and its regulation by IL4 in chronic lymphocytic leukemia, Leukemia Res., 14, pp. 47-55, (1990)
[10] Sarfati M., Bron D., Lagneaux L., Fonteyn C., Frost H., Delespesse G., Elevation of IgE-binding factors in serum of patients with B cell-derived chronic lymphocytic leukemia, Blood, 71, pp. 94-98, (1988)

← 1 2 3 4 →